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Structure and properties of the exopolysaccharides produced by Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014
Abstract
This paper presents a study on the purification, primary structure, and rheological properties of exopolysaccharides isolated from cultures of Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014. Both polymers are exopolysaccharides of D-mannose. The mannan isolated from P. mutabilis T6 contains on average about 5% of residual b-D-glucose, in contrast to the mannan from P. mutabilis ATCC 31014, which contained only trace amounts of residual b-D-glucose (less than 1%). Based on the 13C NMR spectra, all of the remaining carbohydrates in the exopolysaccharides occur in the form of pyranose rings. All of the mannose residues have the a configuration at the anomeric carbon atom while the glucose adopts the b configuration. The reaction of both polysaccharide hydrolysates with an optically active alcohol indicatesthat all of the sugar residues have the D configuration. We found that the main chain of the exopolysaccharide is composed of mannose residues connected through a-(1?6) linkages, of which a large number are substituted on O2 with D-mannose and the remaining are substituted with di- to pentasaccharide fragments. The rheological properties of the exopolysaccharide isolated from P. mutabilis T6 show that its viscosity is over 30 times greater than that of P. mutabilis ATCC 31014.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
CARBOHYDRATE RESEARCH
no. 348,
pages 84 - 90,
ISSN: 0008-6215 - Language:
- English
- Publication year:
- 2012
- Bibliographic description:
- Kuźma M., Clarc B., Ewards J., Tylingo R., Samaszko J.: Structure and properties of the exopolysaccharides produced by Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014// CARBOHYDRATE RESEARCH. -Vol. 348, (2012), s.84-90
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.carres.2011.11.012
- Verified by:
- Gdańsk University of Technology
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