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- 2024
- 2023
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- Gdańsk University of Technology
  Expand the catalog: Gdańsk University of Technology
  - Vice-Rector for Scientific Research
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Plasmids pMET2M1 and pMET15M1 with the SAT1 Flipper knockout cassette construction
Open Research Data
open access
- K. Rząd
- I. Gabriel
The goal of the study was to obtain plasmids containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (MET2, MET15) and to introduce a cleavage site for restriction enzymes into them.
Inserts amplification for knockout cassette construction
Open Research Data
open access
- I. Gabriel
- K. Rząd
The goal of the study was to obtain optimal conditions for amplification of upstream and downstream genes fragments and introduce a cleavage site for restriction enzymes into them thanks to which it will be possible to clone fragments into a plasmid containing elements of the knockout cassette. The constructed cassettes will enable the removal of the...

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