Search results for: BACTERIAL ENDOTOXIN

• Electrochemical detection of bacterial endotoxin lipopolysaccharide (LPS) on gold electrode modified with DAL-PEG-DK5-PEG-OH - Antimicrobial peptide conjugate

  Publication

  • K. Paulina
  • A. Golda
  • J. Ryl
  • M. Pilarczyk-zurek
  • G. Bereta
  • T. Ossowski
  • A. Lesner
  • J. Koziel
  • A. Prahl
  • P. Niedziałkowski

  - TALANTA.The International Journal of Pure and Applied Analytical Chemistry - Year 2024

  This work describes fabrication of gold electrodes modified with peptide conjugate DAL-PEG-DK5-PEG-OH that enables ultra-sensitive detection of lipopolysaccharide (LPS) isolated from the reference strain of Escherichia coli O26:B6. The initial step of the established procedure implies immobilization of the fully protected DAL-PEG-DK5-PEG-OH peptide on the surface of the gold electrode previously modified by cysteamine. Then side...

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• Lipopolysaccharides: regulated biosynthesis and structural diversity

  Publication

  • S. Raina

  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES - Year 2023

  The cell envelope of Gram-negative bacteria contains two distinct membranes, an inner (IM) and an outer (OM) membrane, separated by the periplasm, a hydrophilic compartment that includes a thin layer of peptidoglycan. The most distinguishing feature of such bacteria is the presence of an asymmetric OM with phospholipids located in the inner leaflet and lipopolysaccharides (LPSs) facing the outer leaflet. The maintenance of this...

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