Search results for: PEHX GENE

• DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation

  Publication

  • K. Stojowska
  • B. Krawczyk
  • J. Kur

  - Acta Biochimica Polonica - Year 2011

  A new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...

  Full text to download in external service

• A new assay for the simultaneous identification and differentiation of Klebsiella oxytoca strains.

  Publication

  • K. Stojowska-Swędrzyńska
  • B. Krawczyk

  - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY - Year 2016

  Klebsiella oxytoca is the second most frequently identified species of Klebsiella isolated from hospitalized patients. Klebsiella spp. is difficult to identify using conventional methods and is often misclassified in clinical microbiology laboratories. K. oxytoca is responsible for an increasing number of multi-resistant infections in hospitals because of insufficient detection and identification. In this study, we propose a new...

  Full text available to download