Search results for: heptosyltransferase

• Escherichia coli K-12 suppressor-free mutants lacking early glycosyltransferases and late acyltransferases. Minimal lipopolysaccharide structure and induction of envelope stress response.

  Publication

  • G. Klein-Raina
  • B. Lindner
  • W. Brabetz
  • H. Brade
  • S. Raina

  - JOURNAL OF BIOLOGICAL CHEMISTRY - Year 2009

  To elucidate the minimal lipopolysaccharide (LPS) structure needed for the viability of Escherichia coli, suppressor-free strains lacking either the 3-deoxy-d-manno-oct-2-ulosonic acid transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed. Δ(waaC lpxL lpxM lpxP) and waaA mutants exhibited...

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• Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein

  Publication

  • G. Klein-Raina
  • N. Kobylak
  • B. Lindner
  • A. Stupak
  • S. Raina

  - JOURNAL OF BIOLOGICAL CHEMISTRY - Year 2014

  Here, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...

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