Search results for: FILAGGRIN, EXTRACELLULAR VESICLES, CD1A

• Keratinocyte-derived small extracellular vesicles supply antigens for CD1a-resticted T cells and promote their type 2 bias in the context of filaggrin insufficiency

  Publication

  • A. Kobiela
  • W. Hewelt-Belka
  • J. Frackowiak
  • N. Kordulewska
  • L. Hovhannisyan
  • A. Bogucka
  • R. Etherington
  • A. Piróg
  • I. Dapic
  • S. Gabrielsson... and 3 others

  - Frontiers in Immunology - Year 2024

  Introduction: Exosome-enriched small extracellular vesicles (sEVs) are nanosized organelles known to participate in long distance communication between cells, including in the skin. Atopic dermatitis (AD) is a chronic inflammatory skin disease for which filaggrin (FLG) gene mutations are the strongest genetic risk factor. Filaggrin insufficiency affects multiple cellular function, but it is unclear if sEV-mediated cellular communication...

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• O07 Filaggrin insufficiency renders keratinocyte-derived small extracellular vesicles capable of affecting CD1a-mediated T-cell responses and promoting allergic inflammation

  Publication

  • A. Kobiela
  • W. Hewelt-Belka
  • J. Frackowiak
  • N. Kordulewska
  • L. Hovhannisyan
  • A. Bogucka
  • R. Etherington
  • A. Pirog
  • I. Dapic
  • S. Gabrielsson... and 3 others

  - BRITISH JOURNAL OF DERMATOLOGY - Year 2023

  The association between FLG loss-of-function mutations and the development of atopic dermatitis (AD) indicates that filaggrin is critical to skin barrier function; the mutations are also linked to additional allergic manifestations, but it is unknown how the skin may influence inflammation in distant tissues. Here we investigated the impact of filaggrin insufficiency on keratinocyte-derived exosome-enriched small extracellular...

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• Perspectives and challenges in extracellular vesicles untargeted metabolomics analysis

  Publication

  • D. Dudzik
  • S. Macioszek
  • W. Struck-Lewicka
  • M. Kordalewska
  • M. Buszewska-Forajta
  • M. Waszczuk-Jankowska
  • R. Wawrzyniak
  • M. Artymowicz
  • J. Raczak-Gutknecht
  • D. Siluk
  • M. Markuszewski

  - TrAC Trends in Analytical Chemistry - Year 2021

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• MicroRNA composition of plasma extracellular vesicles: a harbinger of late cardiotoxicity of doxorubicin

  Publication

  • J. Totoń-Żurańska
  • J. Sulicka-Grodzicka
  • M. Seweryn
  • E. Pitera
  • P. Kapusta
  • P. Konieczny
  • L. Drabik
  • M. Kołton-Wróż
  • B. Chyrchel
  • E. Nowak... and 3 others

  - MOLECULAR MEDICINE - Year 2022

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• Isolation of Citrus lemon extracellular vesicles: Development and process control using capillary electrophoresis

  Publication

  • A. Steć
  • M. Chodkowska
  • J. Kasprzyk-Pochopień
  • P. Mielczarek
  • W. Piekoszewski
  • B. Lewczuk
  • A. Płoska
  • L. Kalinowski
  • B. Wielgomas
  • S. Dziomba

  - FOOD CHEMISTRY - Year 2023

  A new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon juice samples was developed. The methodology included preliminary preconcentration of the sample using ultrafiltration (UF) followed by size-exclusion chromatography (SEC) purification and final preconcentration of the eluates. Transmission electron microscopy and proteomic analysis showed that isolates contained exosome-like vesicles, exocyst-positive...

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• Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine

  Publication

  • K. Sałaga-zaleska
  • A. Kuchta
  • B. Bzoma
  • G. Chyła-Danił
  • A. Safianowska
  • A. Płoska
  • L. Kalinowski
  • A. Dębska-ślizień
  • M. Jankowski

  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES - Year 2023

  Urinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein...

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• Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro

  Publication

  • A. Abramowicz
  • A. Wojakowska
  • L. Marczak
  • M. Lysek-Gladysinska
  • M. Smolarz
  • M. Story
  • J. Polanska
  • P. Widlak
  • M. Pietrowska

  - JOURNAL OF RADIATION RESEARCH - Year 2019

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• Proteomic profile of melanoma cell‐derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression

  Publication

  • M. Pietrowska
  • A. Zebrowska
  • M. Gawin
  • L. Marczak
  • P. Sharma
  • S. Mondal
  • J. Mika
  • J. Polańska
  • S. Ferrone
  • J. Kirkwood... and 2 others

  - Journal of Extracellular Vesicles - Year 2021

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• Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis

  Publication

  • A. Steć
  • J. Jońca
  • K. Waleron
  • M. Waleron
  • A. Płoska
  • L. Kalinowski
  • B. Wielgomas
  • S. Dziomba

  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES - Year 2022

  Extracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A satisfactory...

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• Journal of Extracellular Vesicles

  Journals

  ISSN: 2001-3078