Construction of recombinant plasmid coding Borrelia burgdorferi sl B6 protein - Open Research Data - MOST Wiedzy

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Construction of recombinant plasmid coding Borrelia burgdorferi sl B6 protein

Opis

Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and vector (temporal and spatial antigenic variability) has posed challenges for the serodiagnosis of LB. Despite numerous efforts, little is still known about proteins or their fragments which are conserved for all pathogenic genospecies and can be used in the detection of specific immunoglobulins from human serum samples. Therefore, the correct serodiagnosis of this disease still faces many difficulties. For this reason, it is extremely important to conduct basic research aimed at selecting, obtaining and testing the antigenic properties of new forms of recombinant proteins. Thus, the essence of the problem that we intend to solve is to ascertain whether it is possible to obtain new variants of B. burgdorferi sl recombinant proteins which have significant antigenic properties and may detect immunoglobulins from patients’ sera with Lyme disease that may be infected with different genospecies of B. burgdorferi sl. This dataset contains data regarding the construction of a recombinant plasmid coding a Borrelia burgdorferii sl protein named  "B6".

Data includes the methodology, cloning diagrams, primer sequences and nucleotide sequence of the obtained recombinant plasmids.

This research was funded by the National Science Centre, Poland, under the research project no. 2023/49/B/NZ6/02881

Plik z danymi badawczymi

B6.zip
1.1 MB, S3 ETag 1ff9c6881b794008a79ac2e2f6d17f49-1, pobrań: 1
Hash pliku liczony jest ze wzoru
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} gdzie pojedyncza część pliku jest wielkości 512 MB

Przykładowy skrypt do wyliczenia:
https://github.com/antespi/s3md5

Informacje szczegółowe o pliku

Licencja:
Creative Commons: by 4.0 otwiera się w nowej karcie
CC BY
Uznanie autorstwa
Embargo na plik:
2025-03-12

Informacje szczegółowe

Rok publikacji:
2024
Data zatwierdzenia:
2024-12-31
Język danych badawczych:
angielski
Dyscypliny:
  • nauki chemiczne (Dziedzina nauk ścisłych i przyrodniczych)
  • nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu)
DOI:
Identyfikator DOI 10.34808/8b21-n206 otwiera się w nowej karcie
Finansowanie:
Seria:
Weryfikacja:
Politechnika Gdańska

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