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Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants
Abstrakt
This study demonstrates the potential of a commercially available derivatization instrument coupled with HPLC for separation and on-line determination of antioxidants detected with widely used screening reagents - DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) or FCR (Folin-Ciocalteu's phenol reagent ). As the result of optimization, key parameters of the analytical procedure, were established: concentrations of derivatization reagents 1.5 mmol/L and 2.1 mmol/L in methanol for DPPH and ABTS, respectively and 40 % (v/v) FCR solution in water; reactor temperatures 130 oC for ABTS and FCR and 50 oC in the case of DPPH. In order to validate these procedures, Trolox equivalent antioxidant capacities (TEAC) for standard antioxidants obtained by post-column derivatization (on-line) and typical batch colorimetric methods (off-line) were compared; the corresponding measurements were found to be strongly correlated (Pearson coefficient: 0.973, 0.922, 0.853 for DPPH, ABTS and FCR, respectively). The results obtained for standard antioxidants and real fruit sample - aronia extract, confirm the applicability of the proposed system to the on-line detection of antioxidants. Its great advantage compared to current routine methods is that it provides chromatographic profiles and corresponding fingerprints of antioxidants (including unknown ones) along with quantitative determination of antioxidative potential, both - total and exhibited by individual compounds.
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- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuł w czasopiśmie wyróżnionym w JCR
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
nr 24,
wydanie 7,
strony 1073 - 1080,
ISSN: 0889-1575 - Język:
- angielski
- Rok wydania:
- 2011
- Opis bibliograficzny:
- Kusznierewicz B., Piasek A., Bartoszek-Pączkowska A., Namieśnik J.: Application of a commercially available derivatization instrument and commonly used reagents to HPLC on-line determination of antioxidants// JOURNAL OF FOOD COMPOSITION AND ANALYSIS. -Vol. 24, iss. 7 (2011), s.1073-1080
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.jfca.2011.01.010
- Bibliografia: test
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więcej (18) mniej
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- Shi, S.-Y., Zhou, H.-H., Zhang, Y.-P., Jiang, X.-Y., Chen, X.-Q., Huang, K.-L., 2009. Coupling HPLC to on-line, post-column (bio)chemical assays for high-resolution screening of bioactive compounds from complex mixtures. Trends in Analytical Chemistry 28, 865-877. otwiera się w nowej karcie
- Stalmach, A., Mullen, W., Nagai, C., Crozier, A., 2006. On-line HPLC analysis of the antioxidant activity of phenolic compounds in brewed, paper-filtered coffee. Brazilian Journal of Plant Physiology 18, 253-262. otwiera się w nowej karcie
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- Weryfikacja:
- Politechnika Gdańska
wyświetlono 133 razy