Abstrakt

The aerosols generated from e-cigarettes are composed of liquid and gas phases resulting from vapourized e-liquid. The apportioning of substances from e-liquid into the liquid and gas phases during e-cigarette use has not been extensively studied. Partitioning of e-liquid components between the gas and the liquid phase of the aerosol influences the substances inhaled and exhaled by the users, leading to second-hand exposure. It seems important to determine which compounds and how much of them are transferred into the gas phase and may immediately enter the bloodstream. For this purpose, a method based on thermal desorption followed by gas chromatography coupled with tandem mass spectrometry (GC–MS/MS) in electron ionization mode was developed. As in a previous study, an automatic generator of an aerosol from an e-cigarette with a collection tube filled with melt-blown non-woven fabric discs and equipped with Tenax TA sorption tubes was used. The melt-blown non-woven fabric is designed to capture liquid phase compounds, while sorption tubes are meant to sorb compounds in the gas phase of the aerosol. To control the e-liquid mass changes before and after a puff session, quantitation based on the mass change tracking approach (MCT) was applied. Accuracy of the developed method ranged between 91% and 110% regardless of the spiking level, with precision and reproducibility better than 10%. The limits of detection (LODs) ranged from 0.015 to 0.076 ng of substance emitted/mg of consumed e-liquid, while limits of quantitation (LOQs) ranged from 0.045 to 0.23 ng of substance emitted/mg of consumed e-liquid. Most of the compounds are deposited in the liquid phase of the aerosol, while only trace levels of some substances may be observed in an actual, non-condensed gas phase.

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