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Detection of sulfonamide resistance genes via in situ PCR-FISH

Abstrakt

Due to the rising use of antibiotics and as a consequence of their concentration in the environment an increasing number of antibiotic resistant bacteria is observed. The phenomenon has a hazardous impact on human and animal life. Sulfamethoxazole is one of the sulfonamides commonly detected in surface waters and soil. The aim of the study was to detect sulfamethoxazole resistance genes in activated sludge biocenosis by use of in situ PCR and/or hybridization. So far no FISH probes for the detection of SMX resistance genes have been described in the literature. We have tested common PCR primers used for SMX resistance genes detection as FISH probes as well as a combination of in situ PCR and FISH. Despite the presence of SMX resistance genes in activated sludge confirmed via traditional PCR, the detection of the genes via microscopic visualization failed.

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Wersja publikacji
Accepted albo Published Version
Licencja
Creative Commons: CC-BY-NC-ND otwiera się w nowej karcie

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Kategoria:
Publikacja w czasopiśmie
Typ:
artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:
Polish Journal of Microbiology nr 63, strony 167 - 173,
ISSN: 1733-1331
Język:
angielski
Rok wydania:
2014
Opis bibliograficzny:
Gnida A., Kunda K., Ziembińska A., Łuczkiewicz A., Felis E., Surmacz-Górska J.: Detection of sulfonamide resistance genes via in situ PCR-FISH// Polish Journal of Microbiology. -Vol. 63, nr. 2 (2014), s.167-173
Weryfikacja:
Politechnika Gdańska

wyświetlono 122 razy

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