Divulging the anti-acetylcholinesterase activity of Colletotrichum lentis strain KU1 extract as sustainable AChE active site inhibitors

Anoop Kallingal; Aravind Ayyolath; Varun Thachan Kundil; Akshay Maniyeri Suresh; Tomy Muringayil Joseph; Józef Haponiuk; Sabu Thomas; Anu Augustine

doi:10.1007/s00203-022-03288-9

Divulging the anti-acetylcholinesterase activity of Colletotrichum lentis strain KU1 extract as sustainable AChE active site inhibitors

Abstrakt

Alzheimer’s disease (AD), also called senile dementia is a neurodegenerative disease seen commonly in the elderly and is characterised by the formation of β-amyloid plaques and neurofbrillary tangles (NFT). Though a complete understanding of the disease is lacking, recent studies showed the role of the enzyme acetylcholinesterase (AChE) in pathogenesis. Finding new lead compounds from natural sources has always been a quest for researchers. Endophytic fungi are a set of microbes that reside within plants without causing any harm. This study focuses on screening endophytes for the production of active acetylcholinesterase inhibitors. Five endophytic fungi were isolated from Catharanthus roseus and screened for AChE inhibitory activity. Three isolates were found to inhibit AChE inhibitory activity and were distinguished based on molecular and microscopic methods. The mycelial extract was taken for the bioassay-guided column chromatography and TLC was performed on the active fraction. The GC–MS and NMR analysis identifed the active compounds in the extract as 9-hexadecen-1-ol and erucamide. Molecular docking studies revealed that the compounds are thermodynamically feasible and have signifcant glide scores. Computational studies revealed that the hydroxyl group of 9-hexadecen-1-ol forms a hydrogen bond with Ser 293 in the active site of AChE, whereas the active site interactions were predominantly hydrophobic in the case of erucamide and are refected in AChE inhibition assays.

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Autorzy (8)

Anoop Kallingal
Aravind Ayyolath
Varun Thachan Kundil
Akshay Maniyeri Suresh (dawniej: Akshay M S)
Tomy Muringayil Joseph (dawniej: Tomy Muringayil)
Józef Haponiuk prof. dr hab. inż.
Sabu Thomas Profesor
Anu Augustine

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Wersja publikacji: Accepted albo Published Version
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1007/s00203-022-03288-9
Licencja: Copyright (2022 Springer Nature Switzerland AG)

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Kategoria:

Publikacja w czasopiśmie

Typ:

artykuły w czasopismach

Opublikowano w:

ARCHIVES OF MICROBIOLOGY nr 204,
ISSN: 0302-8933

Język:

angielski

Rok wydania:

2022

Opis bibliograficzny:

Kallingal A., Ayyolath A., Thachan Kundil V., M S A., Muringayil T., Haponiuk J., Thomas S., Augustine A.: Divulging the anti-acetylcholinesterase activity of Colletotrichum lentis strain KU1 extract as sustainable AChE active site inhibitors// ARCHIVES OF MICROBIOLOGY -Vol. 204,iss. 12 (2022), s.713-

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