Impact of Brake Wear Particles on Eukaryotic Cell Viability and Associated Oxidative Stress Responses

Lina Trečiokaitė; Yurii Tsybrii; Oleksii Nosko; Lina Ragelienė

doi:10.3390/lubricants12120449

Impact of Brake Wear Particles on Eukaryotic Cell Viability and Associated Oxidative Stress Responses

Abstrakt

In this study, the cytotoxic effects of brake wear particles (≥250 nm ceramic/ceramic wear particles (CCWPs) and ≤100 nm ceramic/steel wear particles (CSWPs)) and 100 nm iron (III) oxide ultrafine particles (IOUFPs) on human lung carcinoma (A549) and Chinese hamster ovary (CHO) cells were investigated. Cell viability was determined using the MTT and Calcein AM methods. Oxidative stress was assessed by measuring reactive oxygen species (ROS), intracellular reduced glutathione (GSH), and malondialdehyde (MDA) concentrations under exposure to the above particles in the concentration range of 10–80 µg/mL. The initial assessments of CCWPs and CSWPs on the cell viability were performed after a 4-h exposure but later extended to 24 h to investigate the time-dependent of the cell viability and oxidative stress. MTT and Calcein AM assays indicated that the A549 cells are less susceptible to CCWPs and CSWPs than the CHO cells when exposed for both 4 h and 24 h. This study highlights that oxidative stress induced by CCWPs, CSWPs, and IOUFPs is cell-specific. While CCWPs did not affect glutathione (GSH) levels in the CHO cells, it significantly reduced GSH levels in A549 cells, with the exception of 80 µg/mL. Both CCWPs and CSWPs increased the lipid peroxidation in both cell types; however, the A549 cells demonstrated lower sensitivity to these treatments.

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Autorzy (4)

Lina Trečiokaitė
- Faculty of Natural Sciences, Vytautas Magnum University, Kaunas, Lithuania
Yurii Tsybrii dr inż.
Oleksii Nosko dr hab. inż.
Lina Ragelienė Doctor of Sciences
- Faculty of Natural Sciences, Vytautas Magnum University, Kaunas, Lithuania

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Wersja publikacji: Accepted albo Published Version
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.3390/lubricants12120449
Licencja: otwiera się w nowej karcie

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Informacje szczegółowe

Kategoria:

Publikacja w czasopiśmie

Typ:

artykuły w czasopismach

Opublikowano w:

Lubricants nr 12,
ISSN: 2075-4442

Język:

angielski

Rok wydania:

2024

Opis bibliograficzny:

Trečiokaitė L., Tsybrii Y., Nosko O., Ragelienė L.: Impact of Brake Wear Particles on Eukaryotic Cell Viability and Associated Oxidative Stress Responses// Lubricants -Vol. 12,iss. 12 (2024), s.449-

DOI: