Wyszukiwarka
Mechanism of recognition of parallel G-quadruplexes by DEAH/RHAU helicase DHX36 explored by molecular dynamics simulations
Abstrakt
Because of high stability and slow unfolding rates of G-quadruplexes (G4), cells have evolved specialized helicases that disrupt these non-canonical DNA and RNA structures in an ATP-dependent manner. One example is DHX36, a DEAH-box helicase, which participates in gene expression and replication by recognizing and unwinding parallel G4s. Here, we studied the molecular basis for the high affinity and specificity of DHX36 for parallel-type G4s using all-atom molecular dynamics simulations. By computing binding free energies, we found that the two main G4-interacting subdomains of DHX36, DSM and OB, separately exhibit high G4 affinity but they act cooperatively to recognize two distinctive features of parallel G4s: the exposed planar face of a guanine tetrad and the unique backbone conformation of a continuous guanine tract, respectively. Our results also show that DSM-mediated interactions are the main contributor to the binding free energy and rely on making extensive van der Waals contacts between the GXXXG motifs and hydrophobic residues of DSM and a flat guanine plane. Accordingly, the sterically more accessible 5′-G-tetrad allows for more favorable van der Waals and hydrophobic interactions which leads to the preferential binding of DSM to the 5′-side. In contrast to DSM, OB binds to G4 mostly through polar interactions by flexibly adapting to the 5′-terminal guanine tract to form a number of strong hydrogen bonds with the backbone phosphate groups. We also identified a third DHX36/G4 interaction site formed by the flexible loop missing in the crystal structure.
Cytowania
-
1 2
CrossRef
-
0
Web of Science
-
1 1
Scopus
Autorzy (4)
-
-
Mateusz Kogut
Cytuj jako
Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- Licencja
-
otwiera się w nowej karcie
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
-
Computational and Structural Biotechnology Journal
nr 19,
strony 2526 - 2536,
ISSN: 2001-0370 - Język:
- angielski
- Rok wydania:
- 2021
- Opis bibliograficzny:
- Hossain K., Jurkowski M., Czub J., Kogut M.: Mechanism of recognition of parallel G-quadruplexes by DEAH/RHAU helicase DHX36 explored by molecular dynamics simulations// Computational and Structural Biotechnology Journal -Vol. 19, (2021), s.2526-2536
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.csbj.2021.04.039
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 164 razy