Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human? - Publikacja - MOST Wiedzy

Twoje konto

zaloguj

Wyszukiwarka

Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human?

Abstrakt

Albert is a dog (Canis familiaris), but he does not realize this. Albert loves human food (and beer), watching movies on the internet, sleeping in bed, and more. But he should not do all these things. To convince him that, we have desinged a test procedure. The DEFB1 gene is unique to human species. Detecting its presence from saliva and in short periods may offer an advantage in the field of forensic medicine, and influence Albert’s bad habits. This study reveals novel utilization of the multisine impedance spectroscopy carried out during potentiodynamic polarization (pDEIS) of the electrode. We have utilized pDEIS to detect DEFB1 gene in collected saliva samples. The detection process was conducted at the boron-doped diamond surfaces functionalized with DEFB1-complimentary oligonucleotide sequence, anchored at the electrode surface. Next, a short-term electrode incubation in presence of target DNA sample allows for achieving DNA hybridization when exposed to human DNA material. The dsDNA orientation vs electrode surface is affected by polarization, and tracked by changes in the electrode kinetics preceded by subtle capacitance dispersion effects. The optimized measurement conditions range between +0.5 and +0.9 V vs Ag|AgCl due to nonspecific DNA adsorption, affecting heterogeneous charge transfer. The DNA hybridization is not achieved in the case of non-complimentary ssDNA originated from any other species subjected to this test. The discussed differences obtained during electrode incubation are supported by the XPS analyses.

Cytowania

  • 2 1

    CrossRef

  • 0

    Web of Science

  • 1 8

    Scopus

Autorzy (10)

Cytuj jako

Pełna treść

pobierz publikację
pobrano 61 razy
Wersja publikacji
Accepted albo Published Version
Licencja
Creative Commons: CC-BY-NC-ND otwiera się w nowej karcie

Słowa kluczowe

Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuły w czasopismach
Opublikowano w:
SENSORS AND ACTUATORS B-CHEMICAL nr 323,
ISSN: 0925-4005
Język:
angielski
Rok wydania:
2020
Opis bibliograficzny:
Niedziałkowski P., Ślepski P., Wysocka J., Chamier-Ciemińska J., Burczyk Ł., Sobaszek M., Wcisło A., Ossowski T., Bogdanowicz R., Ryl J.: Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human?// SENSORS AND ACTUATORS B-CHEMICAL -Vol. 323, (2020), s.128664-
DOI:
Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1016/j.snb.2020.128664
Źródła finansowania:
Weryfikacja:
Politechnika Gdańska

wyświetlono 208 razy

Publikacje, które mogą cię zainteresować

Heterogeneous oxidation of highly boron-doped diamond electrodes and its influence on the surface distribution of electrochemical activity

2019

Chemical-Assisted Mechanical Lapping of Thin Boron-Doped Diamond Films: A Fast Route Toward High Electrochemical Performance for Sensing Devices

2017

New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples.

  • A. Gajda-Walczak,
  • A. Potęga,
  • A. Kowalczyk
  • + 5 autorów
2023

Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications

2023
Meta Tagi