Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory

Anna Brillowska-Dabrowska; Sanne Sogaard Nielsen; Henrik Vedel Nielsen; Maiken Cavling Arendrup

doi:10.3109/13693780903531579

Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory

Abstrakt

We recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%) yielded fungi in culture while the suspected etiologic agents were noted microscopically in 15 (13.8%) that were negative in culture. Fungi were detected by PCR in 37 (33.9%) samples, of which only three were positive in culture. Since the majority of PCR positive but culture negative samples were positive in microscopic examinations, the increased sensitivity was not due to contamination. PCR inhibitors were present in 5% of the samples, but this was overcome by re-running the samples with a 50% reduction of sample DNA. In conclusion, the PCR test performance in the routine setting was excellent and provided a markedly reduced time to diagnosis with a higher sensitivity.

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Wersja publikacji: Accepted albo Published Version
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.3109/13693780903531579
Licencja: Copyright (2010 ISHAM)

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Informacje szczegółowe

Kategoria:: Publikacja w czasopiśmie
Typ:: artykuł w czasopiśmie wyróżnionym w JCR
Opublikowano w:: MEDICAL MYCOLOGY nr 48, strony 828 - 831,
ISSN: 1369-3786
Język:: angielski
Rok wydania:: 2010
Opis bibliograficzny:: Brillowska-Dabrowska A., Nielsen S., Nielsen H., Arendrup M.: Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory// MEDICAL MYCOLOGY. -Vol. 48, nr. nr 6 (2010), s.828-831
DOI:: Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.3109/13693780903531579
Weryfikacja:: Politechnika Gdańska