Year 2024

• Optimized Protocol for RNA Isolation from Penicillium spp. and Aspergillus fumigatus Strains

  Publication

  • A. Siniecka-Kotula
  • M. Mroczyńska-Szeląg
  • A. Brillowska-Dąbrowska
  • L. Holec-Gąsior

  - CURRENT ISSUES IN MOLECULAR BIOLOGY - Year 2024

  Efficient RNA isolation from filamentous fungi is crucial for gene expression studies, but it poses significant technical challenges due to the robust cell walls and susceptibility of RNA to degradation by ribonucleases. This study presents the effectiveness of two RNA isolation protocols for four species of filamentous fungi: Penicillium crustosum, Penicillium rubens, Penicillium griseofulvum, and Aspergillus fumigatus. Both protocols...

  Full text to download in external service

Year 2012

• Molecular detection of Candida krusei

  Publication

  • A. Brillowska-Dąbrowska
  • A. Siniecka-Kotula (formerly: A. Siniecka)

  - International Research Journal of Microbiology - Year 2012

  The species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsicresistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5...

  Full text to download in external service