Year 2021

• System do diagnostyki dermatofitowych zakażeń powierzchniowych oparty na technice Real-Time PCR

  Publication

  • N. Kobylak

  - Year 2021

  Dermatofity należą do blisko spokrewnionej grupy grzybów, które wykazują wysokie powinowactwo do skeratynizowanych tkanek. Cecha ta czyni je odpowiedzialnymi za powierzchniowe grzybice skóry, paznokci oraz włosów. Szacuje się, iż nawet do 20% ludzi na całym świecie dotkniętych jest infekcjami powodowanymi przez dermatofity. Ich leczenie wymaga długotrwałego zastosowania leków przeciwgrzybiczych. W celu dobrania odpowiedniego leczenia...

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Year 2016

• PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum

  Publication

  • N. Kobylak
  • B. Bykowska
  • E. Kurzyk
  • R. Nowicki
  • A. Brillowska-Dąbrowska

  - JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY - Year 2016

  Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of...

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Year 2014

• Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein

  Publication

  • G. Klein-Raina
  • N. Kobylak
  • B. Lindner
  • A. Stupak
  • S. Raina

  - JOURNAL OF BIOLOGICAL CHEMISTRY - Year 2014

  Here, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...

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Year 2013

• Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region.

  Publication

  • G. Klein-Raina
  • S. Muller-Loennies
  • B. Lindner
  • N. Kobylak
  • H. Brade
  • S. Raina

  - JOURNAL OF BIOLOGICAL CHEMISTRY - Year 2013

  It is well established that lipopolysaccharide (LPS) often carries nonstoichiometric substitutions in lipid A and in the inner core. In this work, the molecular basis of inner core alterations and their physiological significance are addressed. A new inner core modification of LPS is described, which arises due to the addition of glucuronic acid on the third heptose with a concomitant loss of phosphate on the second heptose. This...

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