Biotechnological production and purification of Borrelia burgdorferi sl B3 protein - Open Research Data - Bridge of Knowledge

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Biotechnological production and purification of Borrelia burgdorferi sl B3 protein

Description

Borrelia burgdorferi B3 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset contains data regarding the biotechnologial production and purification of a recombinant B. burgdorferii sl protein named  "B3".

Data includes the methodology,  recombinant protein sequences and an image files with results of SDS-PAGE showing recombinant protein expression in E. coli cells and purified protein preparations.

This research was funded by the National Science Centre, Poland, under the research project no. 2023/49/B/NZ6/02881

Dataset file

B3 production and purification.zip
769.2 kB, S3 ETag b47e42b8e9a9b0901d3a0129da9f9932-1, downloads: 1
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hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5

File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
File embargo:
2025-03-12

Details

Year of publication:
2024
Verification date:
2024-12-31
Dataset language:
English
Fields of science:
  • chemical sciences (Natural sciences)
  • health sciences (Medical and Health Sciences )
DOI:
DOI ID 10.34808/dne1-8y19 open in new tab
Funding:
Series:
Verified by:
Gdańsk University of Technology

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