Biotechnological production and purification of Borrelia burgdorferi sl F protein - Open Research Data - Bridge of Knowledge

Search

Biotechnological production and purification of Borrelia burgdorferi sl F protein

Description

Borrelia burgdorferi F protein could be used in the detection of specific immunoglobulins G (IgG) from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset contains data regarding the biotechnologial production and purification of a recombinant B. burgdorferii sl protein named  "F".

Data includes the methodology,  recombinant protein sequence and an image file with results of SDS-PAGE showing recombinant protein expression in E. coli cells and purified protein preparations.

This research was funded by the National Science Centre, Poland, under the research project no. 2023/49/B/NZ6/02881

Dataset file

Production and purification of recombinant Borrelia burgdorferi F protein in E. coli.zip
3.1 MB, S3 ETag dc102930e266ce612a355c9cb765c3a3-1, downloads: 1
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5

File details

License:
Creative Commons: by 4.0 open in new tab
CC BY
Attribution
File embargo:
2026-12-17

Details

Year of publication:
2024
Verification date:
2024-12-31
Dataset language:
English
Fields of science:
  • health sciences (Medical and Health Sciences )
  • chemical sciences (Natural sciences)
DOI:
DOI ID 10.34808/kxrp-bz56 open in new tab
Funding:
Series:
Verified by:
Gdańsk University of Technology

Keywords

Cite as

seen 13 times