The electrochemical response to BDD electrode functionalization with IgG2B receptor

Description

This dataset contains the results of the electrochemical impedance spectroscopy (EIS) measurements of the boron-doped diamond (BDD) electrode functionalization with IgG2B receptor. The functionalized electrode was then used for the electrochemical detection of the SARS-CoV-2 virus S1 protein.

All electrochemical measurements were carried out in a three-electrode system using a potentiostat SP-300 (Biologic) / PalmSens4. The electrolyte was 1 mM K3[Fe(CN)6] and 1 mM K4[Fe(CN)6] in PBS (pH 7.4), deoxidized by passing through a stream of argon prior to measurement. The electrolyte volume was 1 mL. A platinum wire was used as the counter electrode. The reference electrode was a silver chloride electrode with an Ag|AgCl|3M KCl. The working electrode was the functionalised BDD with an electrolyte-exposed surface area of 0.196 cm2. Cyclic voltammetry (CV) measurements were performed from -0.5 to +0.75 V vs. Ag|AgCl|3MKCl with a polarisation rate of 50 mV/s over three cycles. Electrochemical impedance spectroscopy (EIS) measurement was performed for the sample polarised for 30 s to the potential E0’ before the measurement. The measurement was performed in the frequency range from 10 kHz to 0.1 Hz at 11 points per decade. The amplitude of the excitation signal was 10 mV. The procedure details are placed in the metadata.txt file.

The dataset is a part of the results published in the Sensors and Actuators B journal: DOI: 10.1016/j.snb.2022.132427

Dataset file

impedance EIS IGg2B.zip

1.1 MB, S3 ETag 211767548420f2950448ceb2a6071dfb-1, downloads: 59

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Raw data:: Data contained in dataset was not processed.

Details

Year of publication:

2022

Verification date:

2022-08-01

Dataset language:

English

Fields of science:

chemical sciences (Natural sciences)

DOI:

10.34808/f8fn-3365

Funding:

iCovid, impedimetric platform based on boron-doped diamond nanostructures for quantitative analysis of virus-receptor interactions

Verified by:

Gdańsk University of Technology

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