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Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage
Abstract
Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytesafter hydrogen peroxide (H2O2) treatment (250 lM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 lg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H2O2 and AgNP treatments.
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
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FOOD AND CHEMICAL TOXICOLOGY
no. 50,
pages 3352 - 3359,
ISSN: 0278-6915 - Publication year:
- 2012
- Bibliographic description:
- Hudecova A., Kusznierewicz B., Hasplova K., Huk A., Magdolenova Z., Miadokova E., Galova E., Dusinska M.: Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage// FOOD AND CHEMICAL TOXICOLOGY. -Vol. 50, nr. iss. 9 (2012), s.3352-3359
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.fct.2012.06.017
- Verified by:
- Gdańsk University of Technology
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