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Silver nanoparticles induce premutagenic DNA oxidation that can be prevented by phytochemicals from Gentiana asclepiadea
Abstract
Among nanomaterials, silver nanoparticles (AgNPs) have the broadest and most commercial applications due to their antibacterial properties, highlighting the need for exploring their potential toxicity and underlying mechanisms of action. Our main aim was to investigate whether AgNPs exert toxicity by inducing oxidative damage to DNA in human kidney HEK 293 cells. In addition, we tested whether this damage could be counteracted by plant extracts containing phytochemicals such as swertiamarin, mangiferin and homoorientin with high antioxidant abilities. We show that AgNPs (20nm) are taken up by cells and localised in vacuoles and cytoplasm. Exposure to 1, 25 or 100 µg/ml AgNPs leads to a significant dose-dependent increase in oxidised DNA base lesions (8-oxo-7,8-dihydroguanine or 8-oxoG) detected by the comet assay after incubation of nucleoids with 8-oxoG DNA glycosylase. Oxidised DNA base lesions and strand breaks caused by AgNPs were diminished by aqueous and methanolic extracts from both haulm and flower of Gentiana asclepiadea.
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- Accepted or Published Version
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- Copyright (The Author 2012. Published by Oxford University Press)
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
MUTAGENESIS
no. 27,
edition 6,
pages 759 - 769,
ISSN: 0267-8357 - Language:
- English
- Publication year:
- 2012
- Bibliographic description:
- Hudecová A., Kusznierewicz B., Rundén-Pran E., Magdolenová Z., Haąplová K., Rinna A., Fjellsbø L., Kruszewski M., Lankoff A., Sandberg W., Refsnes M., Skuland T., Schwarze P., Brunborg G., Bjøras M., Collins A., Miadoková E., Gálová E., Duąinská M.: Silver nanoparticles induce premutagenic DNA oxidation that can be prevented by phytochemicals from Gentiana asclepiadea// MUTAGENESIS. -Vol. 27, iss. 6 (2012), s.759-769
- DOI:
- Digital Object Identifier (open in new tab) 10.1093/mutage/ges046
- Verified by:
- Gdańsk University of Technology
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