Isolation and characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua)

Robert Tylingo; Szymon Mania; Panek Anna; Rafał Piątek; Roman Pawłowicz

doi:10.4172/2155-952x.1000234

Isolation and characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua)

Abstract

Acid-soluble collagen (ASC) from the fish skin of African catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua) was extracted and characterized. The ASC extraction yield was 75%, 73% and 68%, respectively. The denaturation and melting temperatures of African catfish ASC (29.3°C and 100.0°C) were significantly higher than ASC of Salmon and Baltic cod (20.6°C and 90.5°C; 15.2°C and 86.7°C, respectively), assessed by differential scanning calorimetry. The SDS-PAGE profile showed that each of tested ASC was the type I collagen and consisted of two different α chains, α1 and α2, as well as a β component. The FTIR spectra of all collagens indicate that the overall their chemical compositions are quite similar. The fish skin collagen is easy to prepare and represents a possible resource for use on industrial scale.

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Authors (5)

Robert Tylingo dr hab. inż.
Szymon Mania dr inż.
Panek Anna
- University of Bath Department of Biology and Biochemisty
Rafał Piątek dr hab. inż.
Roman Pawłowicz dr inż.

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DOI:: Digital Object Identifier (open in new tab) 10.4172/2155-952X.1000234
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Category:: Articles
Type:: publikacja w in. zagranicznym czasopiśmie naukowym (tylko język obcy)
Published in:: Journal of Biotechnology & Biomaterials no. 6, edition 2, pages 1 - 6,
ISSN: 2155-952X
Language:: English
Publication year:: 2016
Bibliographic description:: Tylingo R., Mania S., Anna P., Piątek R., Pawłowicz R.. Isolation and characterization of Acid Soluble Collagen from the Skin of African Catfish (Clarias gariepinus), Salmon (Salmo salar) and Baltic cod (Gadus morhua). Journal of Biotechnology & Biomaterials, 2016, Vol. 6, iss. 2, s.1-6
DOI:: Digital Object Identifier (open in new tab) 10.4172/2155-952x.1000234
Verified by:: Gdańsk University of Technology