Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-GRA5 - Open Research Data - Bridge of Knowledge

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Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen SAG1-SAG2-GRA5

Description

The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give the ambiguous results. Moreover, the commercially available diagnostic tests due to its price are not generally applicable in the serodiagnosis of toxoplasmosis in animals, which requires the analysis of a large number of samples. For this reason, many research groups are currently working on new diagnostic tools, which are mainly recombinant antigens. Compared to the native antigens their production is much easier, cheaper, faster and safer. An additional advantage of the recombinant antigens is easier way to standardize assays.
This study presents the construction of an efficient Escherichia coli expression system for the production of recombinant chimeric protein composed of the immunodominant regions of three Toxoplasma gondii antigens SAG1, SAG2 and GRA5. The produced and purified protein is composed of amino acid residues from: 49-311 of the SAG1 antigen, 30-170 of the SAG2 antigen, 26-120 of the GRA5 antigen. In the next part of the work the diagnostic usefulness of obtaining proteins for detection of anti-T. gondii antibodies were evaluated in the IgG ELISA assay and agglutination test using animal sera (e.g. horses, ovine, and pigs) and human serum samples.

Dataset file

pET30 SAG1-SAG2-GRA5 II.dna
71.8 kB, S3 ETag 68228d586c436ffb32620c0a4930ac80-1, downloads: 4
The file hash is calculated from the formula
hexmd5(md5(part1)+md5(part2)+...)-{parts_count} where a single part of the file is 512 MB in size.

Example script for calculation:
https://github.com/antespi/s3md5

File details

License:
Restricted access
This dataset is a part of the patent application. If you are interested, please contact me (bartlomiej.ferra@pg.edu.pl).
Software:
SnapGene Viewer

Details

Year of publication:
2021
Verification date:
2021-03-31
Creation date:
2021
Dataset language:
English
Fields of science:
  • biological sciences (Natural sciences)
  • health sciences (Medical and Health Sciences )
  • Veterinary science (Agricultural sciences)
DOI:
DOI ID 10.34808/0z0s-ed24 open in new tab
Funding:
Ethical papers:
The sera were collected as part of the project entitled "Toxoplasmosis—facts and myths. Educational initiative raising social awareness about the infection with protozoan Toxoplasma gondii", foundation "Our Children", funded from the Civil Initiatives Fund of the Ministry of Labor and Social Policy (FIO 2008, contract No. 813).
Verified by:
Gdańsk University of Technology

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