A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test

Lucyna Holec-Gąsior; Bartłomiej Ferra; Justyna Czechowska; Illia E. Serdiuk; Karol Krzymiński

doi:10.1016/j.diagmicrobio.2017.12.022

A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test

Abstract

Toxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting speciﬁc antibodies in the patient’s sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to secondary antibody (IgG-AL) and SAG2-GRA1-ROP1L chimeric antigen for T. gondii speciﬁc antibodies detection. The CLIA test was compared with conventional ELISA, which was based on the same recombinant antigen and differed only in terms of the detection methodology of immune complexes. The new CLIA assay proved to be more sensitive and better differentiated sera of patients with T. gondii infection from sera of healthy individuals, being a promising alternative to more labor, cost-demanding and less versatile ELISA as screening test in toxoplasmosis diagnostics.

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Authors (5)

Lucyna Holec-Gąsior dr hab. inż.
Bartłomiej Ferra dr inż.
Justyna Czechowska
- Uniwersytet Gdański, Wydział Chemii Katedra Chemii Fizycznej
Illia E. Serdiuk
- Uniwersytet Gdański
Karol Krzymiński
- Uniwersytet Gdański, Wydział Chemii Katedra Chemii Fizycznej

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artykuł w czasopiśmie wyróżnionym w JCR

Published in:

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE no. 91, edition 1, pages 13 - 19,
ISSN: 0732-8893

Language:

English

Publication year:

2018

Bibliographic description:

Holec-Gąsior L., Ferra B., Czechowska J., Serdiuk I. E., Krzymiński K.: A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test// DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. -Vol. 91, iss. 1 (2018), s.13-19

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