Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants
Abstract
The detection of Toxoplasma gondii infection in small ruminants has important significance for public health and veterinary medicine. This study, for the first time, describes the reactivity of four tetravalent chimeric proteins (AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, AMA1-SAG2-GRA1-ROP1, and SAG2-GRA1-ROP1-GRA2) containing immunodominant regions from the AMA1 (apical membrane antigen 1), SAG2 (surface antigen 2), GRA1 (dense granule antigen 1), GRA2 (dense granule antigen 2), and ROP1 (rhoptry antigen 1) with specific IgG antibodies from the sera of small ruminants with the use of an indirect enzyme-linked immunosorbent assay (ELISA). The reactivity of individual chimeric antigens was analyzed in relation to the results obtained in IgG ELISA based on a Toxoplasma lysate antigen (TLA). All chimeric proteins were characterized by high specificity (between 96.39% to 100%), whereas the sensitivity of the IgG ELISAs was variable (between 78.49% and 96.77%). The highest sensitivity was observed in the IgG ELISA test based on the AMA1-SAG2-GRA1-ROP1. These data demonstrate that this chimeric protein can be a promising serodiagnostic tool for T. gondii infection in small ruminants.
Citations
-
7
CrossRef
-
0
Web of Science
-
1 0
Scopus
Authors (3)
Cite as
Full text
- Publication version
- Accepted or Published Version
- License
- open in new tab
Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
Animals
no. 9,
pages 1 - 8,
ISSN: 2076-2615 - Language:
- English
- Publication year:
- 2019
- Bibliographic description:
- Holec-Gąsior L., Ferra B., Grąźlewska W.: Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants// Animals -Vol. 9,iss. 12 (2019), s.1-8
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/ani9121146
- Bibliography: test
-
- Tenter, A.M.; Heckeroth, A.R.; Weiss, L.M. Toxoplasma gondii: From animals to humans. Int. J. Parasitol. 2000, 30, 1217-1258. open in new tab
- Dubey, J.P. Toxoplasmosis in sheep-the last 20 years. Vet. Parasitol. 2009, 163, 1-14. open in new tab
- Dubey, J.P.; Rajendran, C.; Ferreira, L.R.; Martins, J.; Kwok, O.C.; Hill, D.E.; Villena, I.; Zhou, H.; Su, C.; Jones, J.L. High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USA. Int. J. Parasitol 2011, 41, 827-833. open in new tab
- Buxton, D.; Maley, S.W.; Wright, S.E.; Rodger, S.; Bartley, P.; Innes, E.A. Toxoplasma gondii and ovine toxoplasmosis: New aspects of an old story. Vet. Parasitol. 2007, 149, 25-28. open in new tab
- Innes, E.A.; Bartley, P.M.; Maley, S.; Katzer, F.; Buxton, D. Veterinary vaccines against Toxoplasma gondii. Mem. Inst. Oswaldo Cruz 2009, 104, 246-251. open in new tab
- Holec-Gasior, L. Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: Current status of studies. Clin. Vaccine Immunol. 2013, 20, 1343-1351. open in new tab
- Drapała, D.; Holec-Gąsior, L.; Kur, J. New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis. Diagn. Microbiol. Infect. Dis. 2015, 82, 34-39. open in new tab
- Ferra, B.; Holec-Gąsior, L.; Kur, J. A new Toxoplasma gondii chimeric antigen containing fragments of SAG2, GRA1, and ROP1 proteins-impact of immunodominant sequences size on its diagnostic usefulness. Parasitol. Res. 2015, 114, 3291-3299. open in new tab
- Ferra, B.T.; Holec-Gąsior, L.; Gatkowska, J.; Dziadek, B.; Dzitko, K.; Grąźlewska, W.; Lautenbach, D. The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera. PLoS ONE 2019, 14, e0217866. open in new tab
- Holec-Gąsior, L.; Ferra, B.; Drapała, D.; Lautenbach, D.; Kur, J. A new MIC1-MAG1 recombinant chimeric antigen can be used instead of the Toxoplasma gondii lysate antigen in serodiagnosis of human toxoplasmosis. Clin. Vaccine Immunol. 2012, 19, 57-63. open in new tab
- Holec-Gąsior, L.; Ferra, B.; Drapała, D. MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis. Clin. Vaccine Immunol. 2012, 19, 1977-1979. open in new tab
- Ferra, B.; Holec-Gąsior, L.; Kur, J. Serodiagnosis of Toxoplasma gondii infection in farm animals (horses, swine, and sheep) by enzyme-linked immunosorbent assay using chimeric antigens. Parasitol. Int. 2015, 64, 288-294. open in new tab
- Hiszczyńska-Sawicka, E.; Brillowska-Dąbrowska, A.; Dąbrowski, S.; Pietkiewicz, H.; Myjak, P.; Kur, J. High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Exp. Purif. 2003, 27, 150-157. open in new tab
- Hiszczyńska-Sawicka, E.; Kur, J.; Pietkiewicz, H.; Holec, L.; Gąsior, A.; Myjak, P. Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis. Acta Parasitol. 2005, 50, 249-254.
- Holec-Gąsior, L.; Kur, J.; Hiszczynska-Sawicka, E. GRA2 and ROP1 recombinant antigens as potential markers for detection of Toxoplasma gondii-specific immunoglobulin G in human with acute toxoplasmosis. Clin. Vaccine Immunol. 2009, 16, 510-514. open in new tab
- Holec-Gąsior, L.; Ferra, B.; Hiszczyńska-Sawicka, E.; Kur, J. The optimal mixture of Toxoplasma gondii recombinant antigens (GRA1, P22, ROP1) for diagnosis of ovine toxoplasmosis. Vet. Parasitol. 2014, 206, 146-152. open in new tab
- Dziadek, B.; Gatkowska, J.; Brzostek, A.; Dziadek, J.; Dzitko, K.; Dlugonska, H. Toxoplasma gondii: The immunogenic and protective efficacy of recombinant ROP2 and ROP4 rhoptry proteins in murine experimental toxoplasmosis. Exp. Parasitol. 2009, 123, 81-89. open in new tab
- Holec-Gąsior, L.; Dominiak-Górski, B.; Kur, J. First report of seroprevalence of Toxoplasma gondii infection in sheep in Pomerania, northern Poland. Ann. Agric. Environ. Med. 2015, 22, 604-607. open in new tab
- Sources of funding:
- Verified by:
- Gdańsk University of Technology
seen 96 times