Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)

Ewa Kotlarska; Aneta Łuczkiewicz; Marta Pisowacka; Artur Burzyński

doi:10.1007/s11356-014-3474-7

Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)

Abstract

In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n = 84) and 3.05 % (n = 8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

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Authors (4)

Ewa Kotlarska
- Institute of Oceanology of the Polish Academy of Sciences Genetics and Marine Biotechnology Department
Aneta Łuczkiewicz prof. dr hab. inż.
Marta Pisowacka
- . .
Artur Burzyński
- . .

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Keywords

ESCHERICHIA COLI . WASTEWATER TREATMENT PLANT . MARINE OUTFALLS . INTEGRONS . GENE CASSETTES . ANTIBIOTIC RESISTANCE

Details

Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH no. 22, edition 3, pages 2018 - 2030,
ISSN: 0944-1344
Language:: English
Publication year:: 2015
Bibliographic description:: Kotlarska E., Łuczkiewicz A., Pisowacka M., Burzyński A.: Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)// ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH. -Vol. 22, iss. 3 (2015), s.2018-2030
DOI:: Digital Object Identifier (open in new tab) 10.1007/s11356-014-3474-7
Verified by:: Gdańsk University of Technology