Resistance of Escherichia coli and Enterococcus spp. to selected antimicrobial agents present in municipal

Aneta Łuczkiewicz; Ewa Felis; Aleksandra Ziembińska; Anna Gnida; Ewa Kotlarska; Krystyna Olańczuk-Neyman; Joanna Surmacz-Górska

Resistance of Escherichia coli and Enterococcus spp. to selected antimicrobial agents present in municipal

Abstract

In this study, the susceptibility to erythromycin (E) and to trimethoprim/sulfamethoxazole (SXT) among isolates of Enterococcus spp. and Escherichia coli was tested, respectively. Both fecal indicators were detected and isolated from raw(RW) and treated wastewater (TW) as well as from samples of activated sludge (AS) collected in a local wastewater treatment plant (WWTP). Biodiversity of bacterial community in AS was also monitored using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Additionally, distribution of sul1–3 genes conferring sulfonamide resistance was tested among SXT-resistant E. coli. Simultaneously, basic physicochemical parameters and concentrations of eight antimicrobial compounds (belonging to folate pathway inhibitors and macrolides class) were analyzed in RW and TW samples. Six of the selected antimicrobial agents, namely: erythromycin, clarithromycin, trimethoprim, roxithromycin, sulfamethoxazole, and N-acetylsulfamethoxazole were detected in the wastewater samples. Bacterial biodiversity of AS samples were comparable with no relevant differences. Among tested Enterococcus spp., E-resistant isolates constituted 41%. SXT resistance was less prevalent in E. coli with 11% of isolates. The genes conferring resistance to sulfonamides (sul1–3) were detected in SXT-resistant E. coli of wastewater

Authors (7)

Aneta Łuczkiewicz prof. dr hab. inż.
Ewa Felis
- Silesian University of Technology Faculty of Energy and Environmental Engineering
Aleksandra Ziembińska
- Silesian University of Technology Faculty of Energy and Environmental Engineering
Anna Gnida
- Silesian University of Technology Faculty of Energy and Environmental Engineering
Ewa Kotlarska
- Silesian University of Technology Faculty of Energy and Environmental Engineering
Krystyna Olańczuk-Neyman prof. dr hab. inż.
Joanna Surmacz-Górska
- Silesian University of Technology Faculty of Energy and Environmental Engineering

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: JOURNAL OF WATER AND HEALTH no. 11, pages 600 - 612,
ISSN: 1477-8920
Language:: English
Publication year:: 2013
Bibliographic description:: Łuczkiewicz A., Felis E., Ziembińska A., Gnida A., Kotlarska E., Olańczuk-Neyman K., Surmacz-Górska J.: Resistance of Escherichia coli and Enterococcus spp. to selected antimicrobial agents present in municipal // JOURNAL OF WATER AND HEALTH. -Vol. 11, nr. 4 (2013), s.600-612
Verified by:: Gdańsk University of Technology