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Development and Assessment of Regeneration Methods for Peptide-Based QCM Biosensors in VOCs Analysis Applications
Abstract
Cleaning a quartz crystal microbalance (QCM) plays a crucial role in the regeneration of its biosensors for reuse. Imprecise removal of a receptor layer from a transducer’s surface can lead to unsteady operation during measurements. This article compares three approaches to regeneration of the piezoelectric transducers using the electrochemical, oxygen plasma and Piranha solution methods. Optimization of the cleaning method allowed for evaluation of the influence of cleaning on the surface of regenerated biosensors. The effectiveness of cleaning the QCM transducers with a receptor layer in the form of a peptide with the KLLFDSLTDLKKKMSEC-NH2 sequence was described. Preliminary cleaning was tested for new electrodes to check the potential impact of the cleaning on deposition and the transducer’s operation parameters. The effectiveness of the cleaning was assessed via the measurement of a resonant frequency of the QCM transducers. Based on changes in the resonant frequency and the Sauerbrey equation, it was possible to evaluate the changes in mass adsorption on the transducer’s surface. Moreover, the morphology of the QCM transducer’s surface subjected to the selected cleaning techniques was presented with AFM imaging. The presented results confirm that each method is suitable for peptide-based biosensors cleaning. However, the most invasive seems to be the Piranha method, with the greatest decrease in performance after regeneration cycles (25% after three cycles). The presented techniques were evaluated for their efficiency with respect to a selected volatile compound, which in the future should allow reuse of the biosensors in particular applications, contributing to cost reduction and extension of the sensors’ lifetime.
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Authors (6)
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Tomasz Wasilewski
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Weronika Jakubaszek
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Wojciech Kamysz
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/bios12050309
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Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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Biosensors-Basel
no. 12,
ISSN: 2079-6374 - Language:
- English
- Publication year:
- 2022
- Bibliographic description:
- Wasilewski T., Szulczyński B., Dobrzyniewski D., Jakubaszek W., Gębicki J., Kamysz W.: Development and Assessment of Regeneration Methods for Peptide-Based QCM Biosensors in VOCs Analysis Applications// Biosensors-Basel -Vol. 12,iss. 5 (2022), s.309-
- DOI:
- Digital Object Identifier (open in new tab) 10.3390/bios12050309
- Sources of funding:
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- the Young Scientists Program at the Medical University of Gdansk (MTN 664/508/61/71-1414)
- the Medical University of Gdansk statutory grant no. ST-02-0087/07/508
- Polish National Science Centre PRELUDIUM grant no. 2019/33/N/ST5/01517
- Verified by:
- Gdańsk University of Technology
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