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Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants
Abstract
The main aim of this research was to characterize some plants and to determine their similarities and differences, using spectroscopic methods. The interactions of soluble polyphenols of different plants with human serum albumin (HSA) were investigated by 3D-fluorescence. The obtained fluorescence results allow to classify the investigated plants according to their binding properties. The HSA-binding capacities of these plants were the highest for asparagus and correlated with their antioxidant properties. To our knowledge this is the first report showing differences and similarities in these plants, using spectroscopic techniques. The fluorescence spectral methods, which were applied as a powerful tool showing the quenching properties of intrinsic fluorophores in protein molecules in the presence of some plant polyphenols, can contribute in pharmaceutical and food applications.
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Authors (8)
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Yang-Gyu Ku
- Department of Horticulture Industry, Wonkwang University
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Jong-Hyang Bae
- Department of Horticulture Industry, Wonkwang University
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Alma Leticia Martinez-Ayala
- Centro de Desarrollo de Productos Bioticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla,
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Suchada Vearasilp
- Postharvest Technology Research Institute/ Postharvest Technology Innovation Center,
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Pawel Pasko
- Department of Food Chemistry and Nutrition, Medical College, Jagiellonian University,
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Elena Katrich
- The Hebrew University, Institute for Drug Research, School of Pharmacy, Hadassah Medical School,
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Shela Gorinstein
- The Hebrew University, Institute for Drug Research, School of Pharmacy, Hadassah Medical School,
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Keywords
Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
SENSORS AND ACTUATORS B-CHEMICAL
no. 248,
pages 777 - 784,
ISSN: 0925-4005 - Language:
- English
- Publication year:
- 2017
- Bibliographic description:
- Ku Y., Bae J., Martinez-Ayala A., Vearasilp S., Namieśnik J., Pasko P., Katrich E., Gorinstein S.: Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants// SENSORS AND ACTUATORS B-CHEMICAL. -Vol. 248, (2017), s.777-784
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.snb.2017.04.050
- Verified by:
- Gdańsk University of Technology
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