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Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants

Abstract

The main aim of this research was to characterize some plants and to determine their similarities and differences, using spectroscopic methods. The interactions of soluble polyphenols of different plants with human serum albumin (HSA) were investigated by 3D-fluorescence. The obtained fluorescence results allow to classify the investigated plants according to their binding properties. The HSA-binding capacities of these plants were the highest for asparagus and correlated with their antioxidant properties. To our knowledge this is the first report showing differences and similarities in these plants, using spectroscopic techniques. The fluorescence spectral methods, which were applied as a powerful tool showing the quenching properties of intrinsic fluorophores in protein molecules in the presence of some plant polyphenols, can contribute in pharmaceutical and food applications.

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Authors (8)

  • Photo of  Yang-Gyu Ku

    Yang-Gyu Ku

    • Department of Horticulture Industry, Wonkwang University
  • Photo of  Jong-Hyang Bae

    Jong-Hyang Bae

    • Department of Horticulture Industry, Wonkwang University
  • Photo of  Alma Leticia Martinez-Ayala

    Alma Leticia Martinez-Ayala

    • Centro de Desarrollo de Productos Bioticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla,
  • Photo of  Suchada Vearasilp

    Suchada Vearasilp

    • Postharvest Technology Research Institute/ Postharvest Technology Innovation Center,
  • Photo of  Pawel Pasko

    Pawel Pasko

    • Department of Food Chemistry and Nutrition, Medical College, Jagiellonian University,
  • Photo of  Elena Katrich

    Elena Katrich

    • The Hebrew University, Institute for Drug Research, School of Pharmacy, Hadassah Medical School,
  • Photo of  Shela Gorinstein

    Shela Gorinstein

    • The Hebrew University, Institute for Drug Research, School of Pharmacy, Hadassah Medical School,

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Details

Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
SENSORS AND ACTUATORS B-CHEMICAL no. 248, pages 777 - 784,
ISSN: 0925-4005
Language:
English
Publication year:
2017
Bibliographic description:
Ku Y., Bae J., Martinez-Ayala A., Vearasilp S., Namieśnik J., Pasko P., Katrich E., Gorinstein S.: Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants// SENSORS AND ACTUATORS B-CHEMICAL. -Vol. 248, (2017), s.777-784
DOI:
Digital Object Identifier (open in new tab) 10.1016/j.snb.2017.04.050
Verified by:
Gdańsk University of Technology

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