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Evaluation of long‑term immunity and protection against T. gondii after immunization with multivalent recombinant chimeric T. gondii proteins
Abstract
Toxoplasmosis caused by the opportunistic, cosmopolitan protozoan Toxoplasma gondii is one of the most common parasitoses in the world. Although it may prove dangerous or even fatal for immunocompromised individuals, immunoprophylaxis for humans is still nonexistent. Thus, the aim of the current work was to assess the ability of two immunogenic recombinant chimeric T. gondii proteins, SAG2-GRA1-ROP1 (SGR) and SAG1-MIC1-MAG1-GRA2 (SMMG), selected in previous experiments to induce long-lasting immunity when administered with a safe adjuvant. Thus, the determination of immunological parameters and parasite challenge were performed both two weeks after the last boost injection and 6 months postvaccination. Both experimental vaccines triggered specific humoral and cellular responses in immunized C3H/HeOuJ male mice, characterized by the production of specific IgG (IgG1/IgG2a) antibodies in vivo and the synthesis of key Th1/Th2 cytokines by Toxoplasma lysate antigen-stimulated splenocytes in vitro. Although the levels of specific antibodies and cytokine release were in most cases lower six months postimmunization, the protection rates conferred by the vaccination were comparable regardless of the time after the administration of the last vaccine dose. The results indicate that both preparations induce long-lasting immunity, which makes them attractive candidates for further research aimed at boosting their immunogenicity and immunoprotective capacity.
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Authors (7)
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Maciej Chyb mgr
- Uniwersytet Łódzki, Szkoła Doktorska BioMedChem
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Bożena Dziadek
- Uniwersytet Łódzki, Wydział Biologii i Ochrony Środowiska, Katedra Mikrobiologii Molekularnej
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Katarzyna Dzitko dr hab
- Uniwersytet Łódzki, Wydział Biologii i Ochrony Środowiska, Katedra Mikrobiologii Molekularnej
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Bartłomiej Tomasz Ferra dr inż.
- Gdański Uniwersytet Medyczny, Wydział Nauk o Zdrowiu z Instytutem Medycyny Morskiej i Tropikalnej, Instytut Medycyny Morskiej i Tropikalnej, Zakład Parazytologii Tropikalnej
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Malwina Kawka
- Uniwersytet Łódzki, Wydział Biologii i Ochrony Środowiska, Katedra Mikrobiologii Molekularnej
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Justyna Gatkowska
- Uniwersytet Łódzki, Wydział Biologii i Ochrony Środowiska, Katedra Mikrobiologii Molekularnej
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-023-40147-z
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Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
-
Scientific Reports
no. 13,
ISSN: 2045-2322 - Language:
- English
- Publication year:
- 2023
- Bibliographic description:
- Chyb M., Dziadek B., Dzitko K., Ferra B. T., Kawka M., Holec-Gąsior L., Gatkowska J.: Evaluation of long‑term immunity and protection against T. gondii after immunization with multivalent recombinant chimeric T. gondii proteins// Scientific Reports -,iss. 13(1) (2023), s.12976-
- DOI:
- Digital Object Identifier (open in new tab) 10.1038/s41598-023-40147-z
- Sources of funding:
-
- COST_FREE zz
- Verified by:
- Gdańsk University of Technology
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