Microbial diversity of inflamed and noninflamed gut biopsy tissues in inflammatory bowel disease. - Publication - Bridge of Knowledge

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Microbial diversity of inflamed and noninflamed gut biopsy tissues in inflammatory bowel disease.

Abstract

BACKGROUND: Inflammatory bowel disease (IBD) is a chronic gastrointestinal condition without any known cause or cure. An imbalance in normal gut biota has been identified as an important factor in the inflammatory process. METHODS: Fifty-eight biopsies from Crohn's disease (CD, n = 10), ulcerative colitis (UC, n = 15), and healthy controls (n = 16) were taken from a population-based case-control study. Automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphisms (T-RFLP) were used as molecular tools to investigate the intestinal microbiota in these biopsies. RESULTS: ARISA and T-RFLP data did not allow a high level of clustering based on disease designation. However, if clustering was done based on the inflammation criteria, the majority of biopsies grouped either into inflamed or noninflamed groups. We conducted statistical analyses using incidence-based species richness and diversity as well as the similarity measures. These indices suggested that the noninflamed tissues form an intermediate population between controls and inflamed tissue for both CD and UC. Of particular interest was that species richness increased from control to noninflamed tissue, and then declined in fully inflamed tissue. CONCLUSIONS: We hypothesize that there is a recruitment phase in which potentially pathogenic bacteria colonize tissue, and once the inflammation sets in, a decline in diversity occurs that may be a byproduct of the inflammatory process. Furthermore, we suspect that a better knowledge of the microbial species in the noninflamed tissue, thus before inflammation sets in, holds the clues to the microbial pathogenesis of IBD.

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Category:
Articles
Type:
artykuł w czasopiśmie wyróżnionym w JCR
Published in:
INFLAMMATORY BOWEL DISEASES no. 13, pages 675 - 683,
ISSN: 1078-0998
Language:
English
Publication year:
2016
Bibliographic description:
Shadi S., Kotłowski R., Bernstein C., Krause D.: Microbial diversity of inflamed and noninflamed gut biopsy tissues in inflammatory bowel disease.// INFLAMMATORY BOWEL DISEASES. -Vol. 13, nr. 6 (2016), s.675-683
DOI:
Digital Object Identifier (open in new tab) 10.1002/ibd.20101
Verified by:
Gdańsk University of Technology

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