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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
Abstract
The RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional initiation sites (P1 to P5) located distal to EσE-regulated promoter. These promoters are activated in response to unique signals. Out of these P2, P3 and P4 defined major promoters, recognized by RpoN, RpoD and RpoS sigma factors, respectively. Isolation of trans-acting factors, in vitro transcriptional and gel retardation assays revealed that the RpoN-recognized P2 promoter is positively regulated by QseE/F two-component system and NtrC activator, while the RpoD-regulated P3 promoter is positively regulated by Rcs system in response to defects in LPS core biosynthesis, overproduction of certain lipoproteins and by the global regulator CRP. Strains synthesising Kdo2-LA LPS caused up to 7-fold increase in the rpoEP3 activity, which was abrogated in Δ(waaC rcsB). Overexpression of a novel 73 nt sRNA rirA (RfaH interacting RNA) generated by the processing of 5′UTR of the waaQ mRNA induces the rpoEP3 promoter activity concomitant with a decrease in LPS content and defects in the O-antigen incorporation. In the presence of RNA polymerase, RirA binds LPS regulator RfaH known to prevent premature transcriptional termination of waaQ and rfb operons. Excess of RirA could titrate out RfaH causing LPS defects and the activation of rpoE transcription.
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Authors (6)
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Buko Lindner
- Research Center Borstel Leibniz-Center for Medicine and Biosciences
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Full text
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- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1074/jbc.M116.748954
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- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
JOURNAL OF BIOLOGICAL CHEMISTRY
no. 291,
edition 44,
pages 22999 - 23019,
ISSN: 0021-9258 - Language:
- English
- Publication year:
- 2016
- Bibliographic description:
- Klein-Raina G., Stupak A., Biernacka D., Wojtkiewicz P., Lindner B., Raina S.: Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.// JOURNAL OF BIOLOGICAL CHEMISTRY. -Vol. 291, iss. 44 (2016), s.22999-23019
- DOI:
- Digital Object Identifier (open in new tab) 10.1074/jbc.m116.748954
- Verified by:
- Gdańsk University of Technology
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