Naturally occurring mitochondrial DNA heteroplasmy in the MRL mouse

Paweł Sachadyn; Xiang-Ming Zhang; Lise Desquenne Clark; Robert K. Naviaux; Ellen Heber-Katz

doi:10.1016/j.mito.2008.07.007

Naturally occurring mitochondrial DNA heteroplasmy in the MRL mouse

Abstract

The MRL/MpJ mouse is an inbred laboratory strain of Mus musculus, known to exhibit enhanced autoimmunity, increased wound healing, and increased regeneration properties. We report the full-length mitochondrial DNA (mtDNA) sequence of the MRL mouse (Accession # EU450583), and characterize the discovery of two naturally occurring heteroplasmic sites. The first is a T3900C substitution in the TpsiCloop of the tRNA methionine gene (tRNA-Met; mt-Tm). The second is a heteroplasmic insertion of 1-6 adenine nucleotides in the A-tract of the tRNA arginine gene (tRNA-Arg; mt-Tr) at positions 9821-9826. Thelevel of heteroplasmy varied independently at these two sites in MRL individuals. The length of the tRNAArg A-tract increased with age, but heteroplasmy at the tRNA-Met site did not change with age. The finding of naturally occurring mtDNA heteroplasmy in an inbred strain of mouse makes the MRL mouse a powerful new experimental model for studies designed to explore therapeutic measures to alter the cellularburden of heteroplasmy.

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Category:: Articles
Type:: artykuł w czasopiśmie z listy filadelfijskiej
Published in:: MITOCHONDRION no. 8, pages 358 - 366,
ISSN: 1567-7249
Language:: English
Publication year:: 2008
Bibliographic description:: Sachadyn P., Zhang X., Clark L., Naviaux R., Heber-Katz E.: Naturally occurring mitochondrial DNA heteroplasmy in the MRL mouse// MITOCHONDRION. -Vol. 8., nr. iss. 5-6 (2008), s.358-366
DOI:: Digital Object Identifier (open in new tab) 10.1016/j.mito.2008.07.007
Verified by:: Gdańsk University of Technology