Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification

Natalia Kobylak; Barbara Bykowska; Roman Nowicki; Anna Brillowska-Dąbrowska

doi:10.18388/abp.2014_864

Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification

Abstract

Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.

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Authors (4)

Natalia Kobylak mgr
Barbara Bykowska dr n. med.
- GUMed Wydział Lekarski Katedra Wenerologii, Dermatologii i Alergologii
Roman Nowicki prof. dr hab.
- GUMed Wydział Lekarski Katedra Wenerologii, Dermatologii i Alergologii
Anna Brillowska-Dąbrowska dr hab. inż.

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DOI:: Digital Object Identifier (open in new tab) 10.18388/abp.2014_864
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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: Acta Biochimica Polonica no. 62, edition 1, pages 119 - 122,
ISSN: 0001-527X
Language:: English
Publication year:: 2015
Bibliographic description:: Kobylak N., Bykowska B., Nowicki R., Brillowska-Dąbrowska A.: Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification// Acta Biochimica Polonica. -Vol. 62, iss. 1 (2015), s.119-122
DOI:: Digital Object Identifier (open in new tab) 10.18388/abp.2014_864
Verified by:: Gdańsk University of Technology