Diagnostic PCR assay for Microsporum and Trichophyton infections

Anna Brillowska-Dąbrowska; Aleksandra Świerkowska; Ditte Marie Saunte; Maiken Cavling Arendrup

Diagnostic PCR assay for Microsporum and Trichophyton infections

Abstract

We have previously described a highly sensitive 5-hour PCR test for the rapid diagnosis of onychomycosis which detects any dermatophyte and species-identify T. rubrum from patient specimens. We have subsequently developed and evaluated new PCR tests for detection of Trichophyton and Microsporum canis/audouinii infections.58 dermatophyte isolates (21) Microsporum spp. (4 different species), 35 Trichophyton spp (10 different species) and 2 were E. floccosum) and 10 yeast, mould or human DNA controls samples were included. Twenty-five patient specimens randomly selected among routine samples, 10 hair specimens from guinea pig experimentally infected with M. canis and two from un-infected control animals were included. The isolates were identified by microscopy and culture observation. DNA was prepared by a 10-min procedure from patient specimens, guinea pig specimens and fragments of dermatophyte colonies as previously described. Two pairs of primers were designed based on the alignment (VectorNTI, Informax) of dermatophytes rDNA sequences. The presence of specific PCR products of approximately 302 bp and 279 bp, respectively, was examined using electrophoresis.The evaluation of the two PCR tests indicated excellent sensitivity and specificity. Based upon the local epidemiology algorithms can now be designed for rapid and easy PCR-detection of dermatophytosis with a genus identification allowing optimal selection of antifungal treatment.

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Category:: Other
Type:: supllement, wydanie specjalne, dodatek
Published in:: MYCOSES no. 52,
ISSN: 0933-7407
Title of issue:: Mycoses.
Language:: English
Publication year:: 2009
Bibliographic description:: Brillowska-Dąbrowska A., Świerkowska A., Saunte D., Arendrup M.:Diagnostic PCR assay for Microsporum and Trichophyton infections//.-Vol. 52,nr. iss. 1 supplement(2009),
Verified by:: Gdańsk University of Technology