PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum

Natalia Kobylak; Barbara Bykowska; Ewelina Kurzyk; Roman Nowicki; Anna Brillowska-Dąbrowska

doi:10.1111/jdv.13681

PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum

Abstract

Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex Methods Two PCR assays were designed based on differences in the DNA fragment encoding b-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture. Result The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR. Conclusion We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory prax

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Authors (5)

Natalia Kobylak
Barbara Bykowska dr n. med.
- Gdański Uniwersytet Medyczny, Wydział Lekarski Katedra i Klinika Dermatologii, Wenerologii i Alergologii
Ewelina Kurzyk
Roman Nowicki prof. dr hab.
- Gdański Uniwersytet Medyczny, Wydział Lekarski Katedra i Klinika Dermatologii, Wenerologii i Alergologii
Anna Brillowska-Dąbrowska dr hab. inż.

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Category:: Articles
Type:: artykuł w czasopiśmie wyróżnionym w JCR
Published in:: JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY no. 30, edition 10, pages 1819 - 1822,
ISSN: 0926-9959
Language:: English
Publication year:: 2016
Bibliographic description:: Kobylak N., Bykowska, B., Kurzyk E., Nowicki R., Brillowska-Dąbrowska A.: PCR and real-time PCR approaches to the identification of Arthroderma otae species Microsporum canis and Microsporum audouinii/Microsporum ferrugineum// JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY. -Vol. 30, iss. 10 (2016), s.1819-1822
DOI:: Digital Object Identifier (open in new tab) 10.1111/jdv.13681
Verified by:: Gdańsk University of Technology