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Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples
Abstract
Nowadays PCR method is commonly used in molecular diagnostic. However, in many cases PCR is limited, by the presence of inhibitory substances in biological, soil or food samples Efficiency and fidelity of amplification is strongly connected with DNA polymerase and reaction conditions. To meet the requirements of modern diagnostic methods it is essential to seeking for new DNA polymerases with better properties useful in these field. Modifications based on fusion of DNA polymerases with DNA binding proteins is a novel and promising. In my study fusionTaq DNA polymerase showed increasing of resistance to inhibitors from clinical samples (lactoferrin) in comparison to unmodified Taq polymerase.
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- Category:
- Conference activity
- Type:
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Title of issue:
- Diagmol 2015 XVI Conference on Molecular biology in diagnostic of infectious diseases and biotechnology in Memory of Professor Władysłąw J.H. Kunicki-Goldfinger strony 164 - 166
- Language:
- English
- Publication year:
- 2015
- Bibliographic description:
- Śpibida M., Krawczyk B., Olszewski M.: Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples// Diagmol 2015 XVI Conference on Molecular biology in diagnostic of infectious diseases and biotechnology in Memory of Professor Władysłąw J.H. Kunicki-Goldfinger/ ed. 2015 Warszawa: Comittee on Microbiology, 2015, s.164-166
- Verified by:
- Gdańsk University of Technology
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