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Ultrasensitive electrochemical determination of the cancer biomarker protein sPD-L1 based on a BMS-8-modified gold electrode
Abstract
This work describes the modification of a gold electrode with the BMS-8 compound that interacts with the Programmed Death-Ligand 1 (PD-L1), an immune checkpoint protein. The results show that we can confirm the presence of the sPD-L1 in the concentration range of 10−18 to 10−8 M using electrochemical impedance spectroscopy (EIS) with a limit of detection (LOD) of 1.87 × 10−14 M for PD-L1 (S/N = 3.3) and at a concentration of 10−14 M via cyclic voltammetry (CV). Additionally, high-resolution X-ray photoelectron spectroscopy (XPS), contact angle, and surface free energy measurements were applied to confirm the functionalization of the electrode. We investigated the selectivity of the electrode for other proteins: Programmed Death-1 (PD-1), cluster of differentiation 160 (CD160), and B- and T-lymphocyte attenuator (BTLA) at concentrations of 10−8 M. Differentiation between PD-L1 and PD-1 was achieved based on the analysis of the capacitance effect frequency dispersion at the surface of the modified Au electrode with BMS-8 after incubation at various concentrations of PD-L1 and PD-1 proteins in the range of 10−18 to 10−8 M. Significant differences were observed in the heterogeneity of PD-L1 and PD-1. The results of the quasi-capacitance studies demonstrate that BMS-8 strongly and specifically interacts with the PD-L1 protein.
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Authors (11)
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Paweł Niedziałkowski Dr
- Uniwersytet Gdański
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Magdalena Bojko
- Uniwersytet Gdański
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Anna Wcisło dr
- Uniwersytet Gdański
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Marta Spodzieja
- Uniwersytet Gdański
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Katarzyna Magiera Mularz
- Uniwersytet Jagielloński
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Katarzyna Guzik
- Uniwersytet Jagielloński
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Grzegorz Dubin
- Uniwersytet Jagielloński
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Tad A. Holak
- Uniwersytet Jagielloński
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Tadeusz Ossowski prof. dr hab. inż.
- Uniwersytet Gdański
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Sylwia Rodziewicz-Motowidło dr hab.
- Uniwersytet Gdański
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Keywords
Details
- Category:
- Articles
- Type:
- artykuły w czasopismach
- Published in:
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BIOELECTROCHEMISTRY
no. 139,
ISSN: 1567-5394 - Language:
- English
- Publication year:
- 2021
- Bibliographic description:
- Niedziałkowski P., Bojko M., Ryl J., Wcisło A., Spodzieja M., Magiera Mularz K., Guzik K., Dubin G., Holak T., Ossowski T., Rodziewicz-Motowidło S.: Ultrasensitive electrochemical determination of the cancer biomarker protein sPD-L1 based on a BMS-8-modified gold electrode// BIOELECTROCHEMISTRY -Vol. 139, (2021), s.107742-
- DOI:
- Digital Object Identifier (open in new tab) 10.1016/j.bioelechem.2021.107742
- Verified by:
- Gdańsk University of Technology
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