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Virulence analysis and antibiotic resistance of Klebsiella pneumoniae isolates from hospitalised patients in Poland

Abstract

Klebsiella pneumoniae (KP) is a nosocomial pathogen causing difficult-to-treat infections. The presence of virulence genes and antibiotic resistance of 109 KP isolates from hospitalized patients were investigated. Among them, 68.8% were multi-drug resistant (MDR) and 59.6% produced extended-spectrum beta-lactamases (ESBLs). Metallo-β-lactamases (MBLs) were produced by 22% of isolates (mainly from anus), including 16.5% of isolates producing New Delhi metallo-β-lactamase (NDM-1). The genes encoding adhesins (fimH-91.7%, mrkD-96.3%), enterobactin (entB-100%) and yersiniabactin (irp-1-88%) were frequently identified. The genes encoding salmochelin (iroD-9.2%, iroN-7.3%) and colibactin (clbA, clbB-0.9%) were identified rarely. Iron acquisition system-related kfu gene and wcaG gene involved in capsule production were identified in 6.4% and 11% of isolates, respectively. The rmpA gene associated with hypermucoviscosity was present in 6.4% of isolates. In 19.2% of isolates magA gene was detected, specific for K1 capsule serotype, while 22.9% of isolates showed K2 capsule serotype. The rmpA, iroD or iroN genes being diagnostic biomarkers for hypervirulent KP (hvKP) were detected in 16.5% of isolates. We found that 55.5% of hvKP were MDR and produced ESBLs, thus hospital KP isolates pose a serious threat to the healthcare system.

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DOI:
Digital Object Identifier (open in new tab) 10.1038/s41598-023-31086-w
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Category:
Articles
Type:
artykuły w czasopismach
Published in:
Scientific Reports no. 13,
ISSN: 2045-2322
Language:
English
Publication year:
2023
Bibliographic description:
Kot B., Piechota M., Szweda P., Mitrus J., Wicha J., Grużewska A., Witeska M.: Virulence analysis and antibiotic resistance of Klebsiella pneumoniae isolates from hospitalised patients in Poland// Scientific Reports -Vol. 13,iss. 1 (2023),
DOI:
Digital Object Identifier (open in new tab) 10.1038/s41598-023-31086-w
Sources of funding:
  • This research was carried out with the financial support of Siedlce University of Natural Science and Humanities (Scientific Research Project No. 74/20/B)
Verified by:
Gdańsk University of Technology

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