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Characterisation of Escherichia coli isolates from the blood of haematological adult patients with bacteraemia: translocation from gut to blood requires the cooperation of multiple virulence factors
Abstract
The aim of the study was to investigate whether there are unique pathotypes of Escherichia coli capable of transmission from the gastrointestinal tract to the vascular bed. The study included E. coli strains isolated from clinical materials collected from 115 patients suffering from haematologic malignancies diagnosed with bacteraemia. The genotyping techniques established that 89 E. coli isolates from the blood had the same genotype as the E. coli from the patient's bowel. The presence of 21 genes encoding virulence factors typical of various E. coli pathotypes and their relationship with the phylogenetic group was established. One-dimensional analysis showed that the focG gene occurred more frequently in the control bowel group, while the ampicillin-resistant afa/dr E. coli were associated with bacteraemia. Blood isolates with the highest occurrence of virulence factors belonged to pathogenic group B2 and non-pathogenic group A. The co-occurrence of multiple genes encoding papC, sfa, usp and cnf1 virulence factors probably predisposes E. coli to translocation from the gastrointestinal tract to the vascular bed in the group of patients with haematologic malignancies. Based on clustering analysis, dominance of the most virulent strains assigned to the cluster with seven virulence factors encoded by the following genes, papC, sfaD/E, cnf1, usp, agn43, hlyA and iutA, was found. The obtained results enforce the previously proposed concept of bowel-blood translocation and further expand our hypothesis by defining the unique virulence characteristics of E. coli isolates, which predispose them to bowel colonisation or translocation and bacteraemia in this group of patients.
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Authors (6)
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Anna Śledzińska
- Medical University of Gdańsk, Gdańsk, Poland Department of Therapy Monitoring and Pharmacogenetics
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Alfred Samet
- Gdańsk University of Medicine, Gdańsk, Polandoratory Laboratory of Clinical Microbiology
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Bogdan Nowicki
- Medical College, Nashville, TN, USA Department of Obsterics and Gynecology and Department of Microbiology and Ommunology, Mehhary
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Full text
- Publication version
- Accepted or Published Version
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s10096-015-2331-z
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Details
- Category:
- Articles
- Type:
- artykuł w czasopiśmie wyróżnionym w JCR
- Published in:
-
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
no. 34,
pages 1135 - 1143,
ISSN: 0934-9723 - Language:
- English
- Publication year:
- 2015
- Bibliographic description:
- Krawczyk B., Śledzińska A., Szemiako K., Samet A., Nowicki B., Kur J.: Characterisation of Escherichia coli isolates from the blood of haematological adult patients with bacteraemia: translocation from gut to blood requires the cooperation of multiple virulence factors// EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. -Vol. 34, nr. 6 (2015), s.1135-1143
- DOI:
- Digital Object Identifier (open in new tab) 10.1007/s10096-015-2331-z
- Verified by:
- Gdańsk University of Technology
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