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VUV irradiation studies of plasmid DNA in aqueous solution

Abstract

Interactions of VUV light and DNA samples in aqueous solutions are reported. The damage induced by such radiation is quantified by monitoring both loss of supercoiled DNA and formation of single and double strand breaks using agarose gel electrophoresis. Irradiations were performed using synchrotron VUV photons of 130, 150, 170 and 190 nm. VUV irradiation experiments revealed enhanced damage upon irradiation with 170 nm photons as compared with irradiations with photons of 150 nm and 130 nm. Irradiations carried at 190 nm caused the least damage.

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Authors (7)

  • Photo of  Malgorzata Śmialek

    Malgorzata Śmialek

    • The Open University, Department of Physics and Astronomy
  • Photo of  Søren Hoffmann

    Søren Hoffmann

    • University of Aarhus, Institute for Storage Ring Facilities
  • Photo of  M Folkard

    M Folkard

    • Mount Vernon Hospital Gray Cancer Institute
  • Photo of  Kevin Prise

    Kevin Prise

    • Queen's University Belfast, Centre for Cancer Research and Cell Biology,
  • Photo of  David E.g. Shuker

    David E.g. Shuker

    • The Open University, Department of Chemistry and Analytical Sciences,
  • Photo of  N Braithwaite

    N Braithwaite

    • The Open University, Department of Physics and Astronomy
  • Photo of  Nigel J. Mason

    Nigel J. Mason

    • The Open University, Department of Physics and Astronomy

Cite as

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Publication version
Accepted or Published Version
DOI:
Digital Object Identifier (open in new tab) 10.1088/1742-6596/101/1/012020
License
Copyright (2008 IOP Publishing Ltd)

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Details

Category:
Articles
Type:
publikacja w in. zagranicznym czasopiśmie naukowym (tylko język obcy)
Published in:
Journal of Physics : Conference Series no. 101, pages 1 - 9,
ISSN: 1742-6588
Language:
English
Publication year:
2008
Bibliographic description:
Śmialek M., Hoffmann S., Folkard M., Prise K., Shuker D., Braithwaite N., Mason N.. VUV irradiation studies of plasmid DNA in aqueous solution. Journal of Physics : Conference Series, 2008, Vol. 101, , s.1-9
DOI:
Digital Object Identifier (open in new tab) 10.1088/1742-6596/101/1/012020
Verified by:
Gdańsk University of Technology

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