Search results for: SKIN, EPIDERMIS, KERATINOCYTES, EPIDERMAL STEM CELLS, FLOW CYTOMETRY, CELL ISOLATION, CELL CHARACTERIZATION, CELL CULTURE, RT-PCR

• Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry and PCR Methods

  Publication

  • M. Deptula
  • M. Zawrzykraj
  • P. Słonimska
  • M. Pikuła

  - Year 2024

  Epidermal stem cells, located in the skin, together with keratinocytes are transplanted in regenerative therapies, e.g., for the treatment of burns or other wounds. Here, we describe the protocol of their enzymatic isolation from human skin. It includes separation of the epidermis form the dermis by incubation with dispase followed by cell isolation for epidermis by digestion with trypsin. Cell isolated with this method can be...

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• Transcriptional profile of in vitro expanded human epidermal progenitor cells for the treatment of non-healing wounds

  Publication

  • P. Langa
  • A. Wardowska
  • J. Zieliński
  • J. Podolak-Popinigis
  • P. Sass
  • P. Sosnowski
  • K. Kondej
  • A. Renkielska
  • P. Sachadyn
  • P. Trzonkowski
  • M. Pikuła

  - JOURNAL OF DERMATOLOGICAL SCIENCE - Year 2018

  Background Epidermal progenitor cells (EPCs) have been under extensive investigation due to their increasing potential of application in medicine and biotechnology. Cultured human EPCs are used in the treatment of chronic wounds and have recently became a target for gene therapy and toxicological studies. One of the challenges in EPCs culture is to provide a high number of undifferentiated, progenitor cells displaying high viability...

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• Piotr Jasiński prof. dr hab. inż.

  People

  Katedra Inżynierii Materiałów Funkcjonalnych WETI

  Piotr Jasinski obtained MSc in electronics in 1992 from the Gdansk University of Technology (GUT), Poland. Working at GUT, he received PhD in 2000 and DSc in 2009. Between 2001 and 2004 Post Doctoral Fellow at Missouri University of Science and Technology, while between 2008 and 2010 an Assistant Research Professor. Currently is an Associate Professor at Gdansk University of Technology working in the field of electronics, biomedical...

• Flow cytometric sorting and analysis of human epidermal stem cell candidates

  Publication

  • M. Pikuła
  • K. Kondej
  • J. Jaśkiewicz
  • P. Trzonkowski
  • J. Skokowski

  - CELL BIOLOGY INTERNATIONAL - Year 2010

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• Cell Density-Dependent Cytological Stage Profile and Its Application for a Screen of Cytostatic Agents Active Toward Leukemic Stem Cells

  Publication

  • J. J. Lica
  • G. J. Grabe
  • M. Heldt
  • M. Misiak
  • P. Bloch
  • M. Serocki
  • M. Switalska
  • J. Wietrzyk
  • M. Bagiński
  • A. Hellmann... and 2 others

  - STEM CELLS AND DEVELOPMENT - Year 2018

  Proliferation and expansion of leukemia is driven by leukemic stem cells (LSCs). Multidrug resistance (MDR) of LSCs is one of the main reasons of failure and relapses in acute myeloid leukemia (AML) treatment. In this study, we show that maintaining HL-60 at low cell culture density or applying a 240-day treatment with anthrapyridazone (BS-121) increased the percentage of primitive cells, which include LSCs determining the overall...

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• Koło Naukowe CELL - spotkania i prace badawcze

  e-Learning Courses

  • P. Rajchowski

  Miejsce wymiany wiedzy Koła Naukowego CELL działającego przy Katedrze Systemów i Sieci Radiokomunikacyjnych.

• Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line

  Publication

  • B. J. Myśliwiec

  - CELL BIOLOGY INTERNATIONAL - Year 2014

  Increased levels of cyclin D1 and amplification of CCND1 gene occur in many types of cancers. We have followed the expression of cyclin D1 after treatment with doxorubicin with reference to cell death and other possible therapeutic implications. The effect of the treatment on the cell cycle, survival, intracellular level (flow cytometry), and intracellular localization of cyclin D1 (fluorescence microscopy) and expression of...

• CELL STEM CELL

  Journals

  ISSN: 1934-5909 , eISSN: 1875-9777

• Human Induced Pluripotent Stem Cell-Derived Microvesicles Transmit RNAs and Proteins to Recipient Mature Heart Cells Modulating Cell Fate and Behavior

  Publication

  • S. Bobis-Wozowicz
  • K. Kmiotek
  • M. Sekula
  • S. Kedracka-Krok
  • E. Kamycka
  • M. Adamiak
  • U. Jankowska
  • A. Madetko-Talowska
  • M. Sarna
  • M. Bik-Multanowski... and 5 others

  - STEM CELLS - Year 2015

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• A mechanistically approached review upon assorted cell lines stimulated by athermal electromagnetic irradiation

  Publication

  • K. Askaripour
  • A. Żak

  - CELL CYCLE - Year 2023

  The probable influence of electromagnetic irradiation on cancer treatment has been deduced from the interaction of artificial electromagnetic emissions with biological organisms. Nonetheless, the suspected health effects induced by electromagnetic-based technology imply that such a treatment may contaminate the adjacent healthy cells. Thus, gaining mechanistic insights into the problem is required to avoid athermal health hazards....

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