Search results for: IDENTIFICATION, OPPORTUNISTIC PATHOGENS, PCR, RFLP, SCOPULARIOPSIS

• A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans

  Publication

  • P. Filipkowski
  • M. Koziatek
  • J. Kur

  - EXTREMOPHILES - Year 2006

  We report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus radiopugnans (DrpSSB). PCR-derived DNA fragment containing the complete structural gene for DrpSSB protein was cloned and expressed in Escherichia coli. The gene consisting of an open reading frame of 900 nucleotides encodes a protein of 300 amino acids with a calculated...

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• Phenotypic consequences of the LYS4 gene disruption in Candida albicans

  Publication

  • I. Gabriel
  • K. Kur
  • A. Mazur
  • B. Hube
  • S. Milewski

  - MYCOSES - Year 2012

  The main scientific purpose of our studies was to verify the hypothesis that homoaconitase (HA) from Candida albicans, an enzyme catalyzing a second step of the α-aminoadipate pathway (AAP) of L-Lys biosynthesis may become a new target for antifungal chemotherapy. Previous studies indicated that the A. fumigatus mutant lacking the functional lysF gene, encoding HA, exhibited attenuated virulence in a low-dose mouse infection model...

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