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Search results for: Mitochondria
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Synthesis and biological evaluation of novel 3,6- amide and thioamide substituted- 2,3,4,9-tetrahydro-1H-carbazoles for anti-cancer activity
PublicationHerein, we report the synthesis of new compounds with demonstrated anticancer properties based on the 2,3,4,9- tetrahydro-1H-carbazole scaffold. The Fischer indolization method was used to close the heterocyclic motif. The synthesis method’s scope and limitations were thoroughly assessed through a series of experiments. Biological assays revealed that two thioamide compounds exhibited significant anticancer activity against MCF-7,...
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Determination of cyanide ion in urine samples using ion chromatography with pulsed amperometric detection.
PublicationCyanides are widely applied in industrial activity, including gold and silver mining and the production of organic chemicals. HCN is also formed directly from fossil fuel combustion sources from gasoline and diesel vehicles. In natural environment, in plants seeds for example an apple seed, it exists as a cyanogenic glycosides. The amount of cyanide ions originating from food products is however rather small. Larger concentrations...
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Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis
PublicationLow-molecular synthetic fluorophores are convenient tools in bioimaging applications. Several derivatives of Safirinium dyes as well as their reactive N-hydroxysuccinimide (NHS) esters bearing diverse substituents were synthesized and evaluated experimentally in terms of their lipophilicity by means of reverse-phase and immobilized artificial membrane high-performance liquid chromatography. Subsequently, the selected compounds...
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The Unfolded Protein Response: A Double-Edged Sword for Brain Health
PublicationEfficient brain function requires as much as 20% of the total oxygen intake to support normal neuronal cell function. This level of oxygen usage, however, leads to the generation of free radicals, and thus can lead to oxidative stress and potentially to age-related cognitive decay and even neurodegenerative diseases. The regulation of this system requires a complex monitoring network to maintain proper oxygen homeostasis. Furthermore,...
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Polyacrylamide substrate viscosity impact on temozolomide activity in glioblastoma cells by flow cytometry and rheological measurements
Open Research DataDataset includes raw data on cell lines LN-229 and LN-18 treated with temozolomide measured by flow cytometry, rheometry and cell projections. It also includes calculations necessary for creation of figures and conclusions based on those figures in the publication titiled: "Substrate viscosity impairs temozolomide-mediated inhibition of glioblastoma...
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Analiza porównawcza struktury zalążka u Sedum hispanicum L. i Sedum acre L.
PublicationZalążki Sedum hispanicum i S. acre ze względu na budowę klasyfikuje się jako anatropowe. Rozwój zarodka przebiega według typu Caryophyllad - po podziale zygoty powstają dwie komórki, komórka bazalna, która nie ulega podziałom oraz komórka apikalna, z której powstaje cały zarodek właściwy i chalazalne komórki suspensora. Przez cały okres rozwoju zalążka odżywianie zarodka przechodzi od stadium pobierania substancji odżywczych za...
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Targeting of FLT3-ITD kinase contributes to high selectivity of imidazoacridinone C-1311 against FLT3-activated leukemia cells
PublicationDrugs targeting receptor tyrosine kinase FLT3 are of particular interest since activating FLT3-internal tandem duplication (ITD) mutations abundantly occur in fatal acute myeloid leukemias (AMLs). Imidazoacridinone C-1311, a DNA-reactive inhibitor of topoisomerase II, has been previously shown to be a potent and selective inhibitor of recombinant FLT3. Here, we expand those findings by studying its effect on leukemia cells with...
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Caffeine Inhibits Differentiation Of Lung Cancer Stem Cells By Modulating Their Respiratory Metabolism
PublicationIt is ell established that many tumor types contain a fraction of cells, with stem cell-like properties, called cancer stem cells (CSCs), that are resistant to apoptosis induced by therapeutic agents. The presence of CSCs may explain why a standard anticancer treatment, that eliminates only differentiated cancer cells, does not lead to cancer cure.We previously showed the existence of caffeine-sensitive mechanism that controls...
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Antitumor DNA-Damaging C-1748 is a New Inhibitor of Autophagy that Triggers Apoptosis in Human Pancreatic Cancer Cell Lines
PublicationDespite the enormous progress that has been made over the past decades in diagnosis, treatment and prevention of many types of tumors, survival rates in pancreatic cancer still remain poor. Pancreatic cancer is one of the most malignant and chemoresistant tumors and the profound mechanism supporting these phenomena is the constitutively activated prosurvival autophagy. The antitumor 1-nitroacridine derivative C-1748 belongs to...
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Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction
PublicationBiogenesis of iron–sulfur clusters (FeS) is a highly conserved process involving Hsp70 and J-protein chaperones. However, Hsp70 specialization differs among species. In most eukaryotes, including Schizosaccharomyces pombe, FeS biogenesis involves interaction between the J-protein Jac1 and the multifunctional Hsp70 Ssc1. But, in Saccharomyces cerevisiae and closely related species, Jac1 interacts with the specialized Hsp70 Ssq1,...
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Indukcja apoptozy w komórkach białaczek ludzkich molt4 i hl60 przez acyloksytriazoloakrydony - nową grupe związków o właściwościach przeciwnowotworowych
PublicationWykazazno, że acyloksytriazoloakrydony, podobnie jak badane wcześniej triazoloakrydony, indukują apoptozę w komórkach białaczek ludzkich MOLT4 i HL60 w stężeniach biologicznie istotnych, odpowiadających wartościom EC90 i EC80. Zjawisko apotozy zbadano w oparciu o zmiany morfologiczne w komórkach (ciałka apoptotyczne), międzynukleosomalną fragmentację DNA, zmiany w budowie błony cytoplazmatycznej, spadek mitochondrialnego potencjału...