Search results for: methionine biosynthesis

• Inserts amplification for knockout cassette construction

  Open Research Data

  open access

  • I. Gabriel
  • K. Rząd

  The goal of the study was to obtain optimal conditions for amplification of upstream and downstream genes fragments and introduce a cleavage site for restriction enzymes into them thanks to which it will be possible to clone fragments into a plasmid containing elements of the knockout cassette. The constructed cassettes will enable the removal of the...

• Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP

  Publication

  • H. Cieśliński
  • P. Bartasun
  • A. Bujacz
  • A. Wierzbicka-Woś
  • J. Kur

  - Acta Biochimica Polonica - Year 2011

  In the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...

• Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.

  Open Research Data

  open access

  • I. Gabriel
  • A. Kuplińska
  • K. Rząd
  • S. Milewski

  Enzymes of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p), O-acetylhomoserine sulfhydrylase (Met15p) and cystathionine-γ-synthase (Str2p) could be exploited as molecular targets for antifungal chemotherapy. The goal of the study was to identify and clone genes encoding mentioned above enzymes. MET2, MET15 and STR2 genes...