Search results for: TAQ POLYMERASE

• Polymers

  Journals

  ISSN: 2073-4360

• Increased concentration of Taq DNA polymerase as a solution for GC-rich templates from clinical and environmental samples

  Publication

  • M. Skwarecka
  • M. Olszewski
  • B. Krawczyk

  - Acta Biochimica Polonica - Year 2015

  DNA polymerase is an enzyme which plays crucial role in replication and DNA repair. It found application in PCR (polymerase chain reaction) where catalyses process of in vitro DNA synthesis. To meet the demands posed by mod- ern diagnostic, molecular biology or genetic engineering it is necessary to improve DNA polymerases to obtain new or better features useful in these fields. So far implemented modifications in majority are...

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• Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples

  Publication

  • M. Skwarecka
  • B. Krawczyk
  • M. Olszewski

  - Year 2015

  Nowadays PCR method is commonly used in molecular diagnostic. However, in many cases PCR is limited, by the presence of inhibitory substances in biological, soil or food samples Efficiency and fidelity of amplification is strongly connected with DNA polymerase and reaction conditions. To meet the requirements of modern diagnostic methods it is essential to seeking for new DNA polymerases with better properties useful in these field....

• CARBOHYDRATE POLYMERS

  Journals

  ISSN: 0144-8617 , eISSN: 1879-1344

• Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization

  Publication

  • M. Olszewski
  • M. Skwarecka
  • M. Bilek
  • B. Krawczyk

  - PLOS ONE - Year 2017

  DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR. Modern diagnostics, molecular biology and genetic engineering need DNA polymerases which demonstrate improved performance. This study was aimed at obtaining a new NeqSSB-TaqS fusion DNA polymerase from the...

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• JOURNAL OF POLYMERS AND THE ENVIRONMENT

  Journals

  ISSN: 1566-2543 , eISSN: 1572-8900

• Fuzyjne polimerazy DNA – otrzymywanie, charakterystyka i zastosowanie

  Publication

  • M. Skwarecka

  - Year 2018

  Obecnie reakcje PCR (ang. Polymerase Chain Reaction) wykazują bardzo szerokie zastosowanie w diagnostyce medycznej, biologii molekularnej czy inżynierii genetycznej. Efektywność tych reakcji rozumiana jako wydajność i wierność przeprowadzonej amplifikacji jest nieodłącznie związana ze stosowaną polimerazą DNA i warunkami prowadzenia reakcji PCR. Aby sprostać wymaganiom stawianym przez nowoczesne metody diagnostyczne oraz współczesną...

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• REACTIVE & FUNCTIONAL POLYMERS

  Journals

  ISSN: 1381-5148 , eISSN: 1873-166X

• Modified DNA polymerases for PCR troubleshooting

  Publication

  • M. Skwarecka
  • B. Krawczyk
  • M. Olszewski
  • J. Kur

  - JOURNAL OF APPLIED GENETICS - Year 2017

  PCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase...

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• E-POLYMERS

  Journals

  ISSN: 1618-7229