Abstrakt

This investigation has been carried out to study Micropropagation of Gardenia jasminoides Ellis shootlet,
induction and growth of callus and enhancement of antioxidant, total phenolics and flavonoids content in shootlet and
calli. The results revealed that for shooting stage adding 2 or 3 mg/l BAP gave the highest shootlet number/explant. But
adding 3 mg/l 2iP produced the longest shootlet and leaves number. The highest fresh and dry weight was recorded on
medium free hormones. Antioxidant activity, total phenolics and flavonoids content were highest in shootlet cultured in all
concentrations of cytokinins compared to medium free hormones and mother plants which gave the lowest amount.
Results indicated a strong relationship between total phenolics content and antioxidant activity. On rooting behaviour, all
strengths of MS-medium with 1g/l AC produced rooted shootlet. Quarter strength of MS-medium produced the highest
rooting, leaf number and shootlet length. Rooted shootlets cultured on peatmoss with sand (1:1 v/v) gave the longest
plantlet and greatest number of leaves. For induction and growth callus, adding 2 mg/l NAA recorded the highest
percentage, fresh and dry weight of callus.

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