Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions. - Publikacja - MOST Wiedzy

Wyszukiwarka

Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.

Abstrakt

In this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties. However, the differences concerned the thermal activity were observed. The optimal temperature for recombinant esterase in the presence of urea (1M) was about 15°C lower in comparison with enzyme isolated from the native source. Furthermore, the EstA was found to be more thermolabile in denaturant conditions. The differences were presumably caused by slightly changed protein structure in the presence of urea. The preservation of activity of EstA dissolved in buffer containing 8M urea suggests that the protein structure is retained and it does not undergo dramatic changes due to high urea concentration. This thesis was confirmed with the FT-IR data.

Cytuj jako

Słowa kluczowe

Informacje szczegółowe

Kategoria:
Publikacja w czasopiśmie
Typ:
artykuł w czasopiśmie indeksowanym TR Master Journal List
Opublikowano w:
Polish Journal of Microbiology nr 58, strony 211 - 218,
ISSN: 1733-1331
Język:
angielski
Rok wydania:
2009
Opis bibliograficzny:
Długołęcka A., Cieśliński H., Bruździak P., Gottfried K., Turkiewicz M., Kur J.: Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.// Polish Journal of Microbiology. -Vol. 58., nr. nr 3 (2009), s.211-218
Weryfikacja:
Politechnika Gdańska

wyświetlono 88 razy

Publikacje, które mogą cię zainteresować

Meta Tagi