Wyszukiwarka
A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis
Abstrakt
The collection of 146 Staphylococcus epidermidis strains isolated from the nasopharynx of lung cancer patients has been studied for the ability of slime secretion and biofilm formation using the Congo red agar (CRA) test and the microtiter plate (MtP) method, respectively. The prevalence of the icaAD and the aap genes was also analyzed. Some isolates (35.6%) were biofilm positive by the MtP method, while 58.9% of isolates exhibited a slime-positive phenotype by the CRA test. The sensitivities of the CRA test evaluated using the MtP method as a gold standard of biofilm production were 73.1%, 97.3% and 13.3% for all the strains screened, ica-positive and ica-negative strains, respectively. The genotype ica(+)aap(+) was correlated with a strong biofilm-producer phenotype. Interestingly, some of the ica(-)aap(-) isolates could also form a biofilm. The correlation between the presence of icaAD genes and the biofilm-positive phenotype by the MtP method as well as slime production by the CRA test was statistically significant (P<0.0001). However, some S. epidermidis strains possess the potential ability of ica-independent biofilm formation; thus, further studies are needed to determine reliable, short-time criteria for an in vitro assessment of biofilm production by staphylococci.
Cytowania
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2 6
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0
Web of Science
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3 1
Scopus
Autorzy (8)
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Renata Los
- Department of Pharmaceutical Microbiology, Medical University of Lublin
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Rafal Sawicki
- Department of Biochemistry and Biotechnology, Medical University of Lublin
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Marek Juda
- Department of Pharmaceutical Microbiology, Medical University of Lublin
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Magdalena Stankevic (dawniej: Magdalena Stankevic) dr inż.
- Department of Biochemistry and Biotechnology, Medical University of Lublin
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Pawel Rybojad
- Department of Thoracic Surgery, Medical University of Lublin
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Marek Sawicki
- Department of Thoracic Surgery, Medical University of Lublin
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Anna Malm
- Department of Pharmaceutical Microbiology, Medical University of Lublin
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Grazyna Ginalska
- Department of Biochemistry and Biotechnology, Medical University of Lublin
Cytuj jako
Pełna treść
- Wersja publikacji
- Accepted albo Published Version
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1111/j.1574-6968.2010.02050.x
- Licencja
- Copyright (2010 Federation of European Microbiological Societies)
Słowa kluczowe
Informacje szczegółowe
- Kategoria:
- Publikacja w czasopiśmie
- Typ:
- artykuły w czasopismach
- Opublikowano w:
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FEMS MICROBIOLOGY LETTERS
nr 310,
strony 97 - 103,
ISSN: 0378-1097 - Język:
- angielski
- Rok wydania:
- 2010
- Opis bibliograficzny:
- Los R., Sawicki R., Juda M., Stankevic M., Rybojad P., Sawicki M., Malm A., Ginalska G.: A comparative analysis of phenotypic and genotypic methods for the determination of the biofilm-forming abilities of Staphylococcus epidermidis// FEMS MICROBIOLOGY LETTERS -Vol. 310,iss. 2 (2010), s.97-103
- DOI:
- Cyfrowy identyfikator dokumentu elektronicznego (otwiera się w nowej karcie) 10.1111/j.1574-6968.2010.02050.x
- Weryfikacja:
- Politechnika Gdańska
wyświetlono 109 razy
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